FIGURE

Fig. 5

ID

ZDB-FIG-140602-16

Publication

Zannino et al., 2014 - prdm12b specifies the p1 progenitor domain and reveals a role for V1 interneurons in swim movements

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

prdm12b Defines the dorsal boundary of nkx6.1 expression and regulates the size of the p1 domain. (A–N) Expression of dbx1a/nkx6.1 (A, H), pax3/nkx6.1 (B, I), evx1/nkx2.2 (C, J), pax3/nkx2.2 (D, K), vsx2/nkx2.2 (E, L), irx3/nkx2.2 (F, M) and irx3/olig2 (G, N) in wild-type (A–G) or PTB MO-injected (H–N) 24 hpf embryos. Panels represent cross sections through the hindbrain with dorsal to the top. (O) Quantification of dorsoventral patterning defects using ~100 sections from 16 embryos for each gene pair. (P) Diagram of dorsoventral gene expression in wild type (left half) and prdm12b MO-injected (right half) embryos. (Q) Quantification of the distance between various gene expression domains using 10 representative sections for each gene pair. Arrows indicate ectopic nkx6.1⁺ cells and brackets indicate unlabeled tissue between markers. Scale bar is 100 μm.

Expression Data

Genes:	dbx1a evx1 irx3a nkx2.2a nkx6.1 olig2 pax3a vsx2
Fish:	WT WT + MO1-prdm12b
Knockdown Reagent:	MO1-prdm12b
Anatomical Terms:	hindbrain dorsal region hindbrain dorso-medial region hindbrain lateral region hindbrain ventral region hindbrain ventro-lateral region hindbrain ventro-medial region
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-prdm12b
Knockdown Reagent:	MO1-prdm12b
Observed In:	hindbrain interneuron neuron fate specification
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 390, Zannino, D.A., Downes, G.B., Sagerström, C.G., prdm12b specifies the p1 progenitor domain and reveals a role for V1 interneurons in swim movements, 247-60, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.