FIGURE

Fig. S5

ID

ZDB-FIG-140430-17

Publication

Al Oustah et al., 2014 - Dynamics of Sonic hedgehog signaling in the ventral spinal cord are controlled by intrinsic changes in source cells requiring Sulfatase 1

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Fig. S5

Shh signaling activity is reduced in sulf1-depleted zebrafish embryos. (A-F) Expression of ptch2 at 16hpf (A,D), 24hpf (B,E) and 36hpf (C,F) in embryos injected with ctrlMO (A-C) and sulf1MO (D-F). (G,H) Expression of ptch2 at 24hpf in wild-type (G) and sulf1^sa199-/- (H) embryos. Note reduction in ptch2 expression both in sulf1MO-injected and sulf1^sa199-/- embryos at 24hpf. Scale bar = 20μm.

Expression Data

Gene:	ptch2
Fish:	WT sulf1^sa199/sa199 WT + MO5-sulf1
Knockdown Reagent:	MO5-sulf1
Anatomical Terms:	neural tube spinal cord
Stage Range:	14-19 somites to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sulf1^sa199/sa199 WT + MO5-sulf1
Knockdown Reagent:	MO5-sulf1
Observed In:	neural tube smoothened signaling pathway spinal cord smoothened signaling pathway
Stage Range:	14-19 somites to Prim-25

Phenotype Detail

Acknowledgments

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