FIGURE

Fig. 6

ID

ZDB-FIG-140325-49

Publication

Wylie et al., 2014 - Post-transcriptional regulation of wnt8a is essential to zebrafish axis development

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Fig. 6

UTR2 TP MO elevates wnt8a levels. (A–F) In situ hybridizations, 60–80% epiboly. Probe indicated in upper right, relative probe position is shown in Fig. 1. (A, C, and E) Control morpholino injected. (B,D,F) UTR2 TP MO injected. Note increased staining for wnt8a.1 and wnt8a.2. Insets in A and B: Close-up comparison to highlight differences in wnt8a.1. (G) RT-qPCR quantification of expression changes. Y-axis indicates fold change from levels in control injected embryos. Box indicates ±standard deviation, line in box represents mean. Asterisks indicate p<0.01. (H) Lateral views of live 24 hpf embryos. Treatment indicated above each image. (I) Percent of embryos per class after morpholino co-injection. Number in parentheses=n for each treatment.

Expression Data

Genes:	lef1 wnt8a
Fish:	WT WT + MO7-wnt8a
Knockdown Reagent:	MO7-wnt8a
Anatomical Term:	margin
Stage:	Shield

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-wnt8a + MO2-wnt8a WT + MO1-wnt8a + MO2-wnt8a + MO7-wnt8a WT + MO7-wnt8a
Knockdown Reagents:	MO1-wnt8a MO2-wnt8a MO7-wnt8a
Observed In:	extension eye head whole organism
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 386(1), Wylie, A.D., Fleming, J.A., Whitener, A.E., and Lekven, A.C., Post-transcriptional regulation of wnt8a is essential to zebrafish axis development, 53-63, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.