FIGURE

Fig. 3

ID

ZDB-FIG-140325-45

Publication

Wylie et al., 2014 - Post-transcriptional regulation of wnt8a is essential to zebrafish axis development

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Fig. 3

UTR1 and UTR2 regulation occurs during gastrulation. (A and B) In situ hybridizations to EGFP transcripts in 80% epiboly transgenic embryos, dorsal views, anterior up. Insets: vegetal views, dorsal to right. Arrows indicate ventral margin: note absence of staining at ventral margin in (A) but not (B). (C and D) ~70% epiboly. Note that wnt8a is not normally absent from the ventral margin at this stage. All embryos are from transgenic lines expressing reporters under the 2.8 kb wnt8a promoter; genotypes are abbreviated for simplicity. Brackets indicate the extent of staining away from the embryonic margin. Note the significant expansion of transcripts away from the margin for reporters bearing the reverse complement UTR sequences (B and D) in comparison to the forward orientation (A and C).

Expression Data

Gene:	EGFP
Fish:	x42Tg x43Tg x44Tg x45Tg
Anatomical Terms:	anatomical structure margin margin ventral region
Stage:	75%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 386(1), Wylie, A.D., Fleming, J.A., Whitener, A.E., and Lekven, A.C., Post-transcriptional regulation of wnt8a is essential to zebrafish axis development, 53-63, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.