ZFIN ID: ZDB-FIG-140320-4
Ekker et al., 1992 - Coordinate embryonic expression of three zebrafish engrailed genes. Development (Cambridge, England)   116:1001-1010
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 5-9 somites to Prim-15

Fig. 6 Cells at the junction between the midbrain and hindbrain express all three engrailedgenes. Parasagittal sections through the heads of 12 h (A-C) and 32 h (E-G) embryos and 22 h (D) and 32 h (H) whole-mount embryos were hybridized with probes for the eng1 (A,E), eng2 (8,F) or eng3 (C,D,G,H) genes. A cartoon of the engrailed expression domains at the midbrain/hindbrain junction is shown in (I). The domain expressing only eng3 is shown in blue, the domain expressing both eng2 and eng3 is shown in red, and the domain expressing all three is shown in green. Anterior is oriented to the left and dorsal to the top in this and subsequent figures. AC and E-G are double exposures with bright field (blue) and clarkfield (red) illumination. The red signal in the lower part of I A is due to the yolk, rather than specific hybridization, as shown in control sections without hybridization. Arrows in H indicate hindbrain cells expressing eng3 (see Fig. 8). Scale bar, 80 μm in A-D, 50 μm in E-G and I, 100 μm in H.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
en1a AB standard conditions 5-9 somites midbrain hindbrain boundary neural rod ISH
Prim-15 midbrain hindbrain boundary ISH
en2a AB standard conditions 5-9 somites midbrain hindbrain boundary neural rod ISH
Prim-15 cerebellum ISH
Prim-15 midbrain posterior-most region ISH
Prim-15 midbrain hindbrain boundary ISH
en2b AB standard conditions 5-9 somites midbrain hindbrain boundary neural rod ISH
26+ somites midbrain hindbrain boundary ISH
Prim-15 cerebellum ISH
Prim-15 midbrain posterior region ISH
Prim-15 midbrain hindbrain boundary ISH
Prim-15 rhombomere 1 ISH
Prim-15 rhombomere 2 ISH
Prim-15 rhombomere 3 ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.