FIGURE

Fig. 4

ID

ZDB-FIG-140314-20

Publication

Rodaway et al., 1999 - Induction of the mesendoderm in the zebrafish germ ring by yolk cell-derived TGF-ß; family signals and discrimination of mesoderm and endoderm by FGF

Other Figures

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Fig. 4

gata5 and ntl coexpress in the blastoderm margin. (A) Twocolour whole-mount in situ of a 50% epiboly embryo showing gata5 (blue) expression in the margin of the blastoderm, and wider expression of ntl (red). Scale bar,150 μm. (B-E) sequential in situ detection on the same sections of gata5 (blue; B and C) and ntl (red fluorescence; D and E), at 30% epiboly (B,D) and 50% epiboly (C,E). (B) The initial expression of gata5 in the blastoderm extends 2-3 cell diameters from the blastoderm margin. Expression is also detected in the YSL (white arrow). (C) At 50% epiboly, gata5 expresses for approximately the same distance from the blastoderm margin, which now represents 3-4 cell diameters. (D) Initially ntl expresses in the same blastomeres as gata5, but not in the YSL. (E) 50% epiboly. ntl expression has spread to encompass blastomeres within 8-10 cell diameters of the margin. Thus, by the beginning of gastrulation, gata5-expressing cells are a marginal subset of those expressing ntl.

Expression Data

Genes:	gata5 tbxta
Fish:	WT
Anatomical Terms:	margin yolk syncytial layer
Stage:	50%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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