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ZFIN ID: ZDB-FIG-140314-25
Rodaway et al., 1999 - Induction of the mesendoderm in the zebrafish germ ring by yolk cell-derived TGF-ß; family signals and discrimination of mesoderm and endoderm by FGF. Development (Cambridge, England)   126(14):3067-3078
Anatomical Terms:
Stage Range: 30%-epiboly to Shield

Fig. 9 gata5 expression phenotypes obtained from a cross of fish doubly heterozygotic for squint (sqt cz35) and cyclops (cycm294). Expression was assayed at 30-40% epiboly (A-C, left embryos oblique animal pole view, right embryos lateral view) and shield stage (D-F, animal-lateral view). A and D show the normal expression pattern seen in most embryos. (A) At 30-40% epiboly, expression is in the margin of the blastoderm (open arrowhead) and the underlying YSL (black arrowhead). At shield stage (D) gata5 is expressed in the newly involuted hypoblast. One abnormal phenotype (B and E) was the same as that found in embryos from crosses of sqt single heterozygotes. At 30-40% epiboly (B), expression of gata5 in the blastoderm margin was generally weaker (black arrows) and in some embryos expression was absent from part of the blastoderm margin (white arrowheads). At shield stage (E), expression was absent from typically one quarter of the hypoblast (white arrowheads), and reduced in the remainder. In the second abnormal phenotype, representing embryos homozygous for both sqtcz35 and cycm294, gata5 was completely absent from blastomeres at both stages (C and F). Some remaining expression could be seen in the YSL (C, black arrowhead).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
gata5 WT standard conditions 30%-epiboly margin ISH
Shield margin ISH
ndr1cz35/cz35 standard conditions 30%-epiboly margin ISH
Shield margin ISH
ndr1cz35/cz35; ndr2m294/m294 standard conditions 30%-epiboly Not Detected margin ISH
30%-epiboly yolk syncytial layer ISH
Shield Not Detected margin ISH
Antibody Labeling Details No data available
Phenotype Details No data available
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