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Expression of zebrafish gata5 detected by in situ hybridisation. Stages according to Kimmel et al. (1995). In A and B the animal pole is up and in C-F anterior is to the left and dorsal is up. (A) 40% epiboly. gata5 expression in the germ ring. (B) 80% epiboly. gata5-expressing cells have involuted and dispersed towards the animal pole. (C) 2 somites. gata5-expressing cells are in the lateral mesoderm anteriorly, and in presumptive endoderm cells converging towards the midline. (D) 11 somites. The anterior extent of the expressing cells is beneath the midbrain. (E) 22 somites. The heart is expressing gata5 (arrowheads). Caudally, expressing cells are present around the yolk cell extension (arrows). (F) 24 hours. The arrowhead marks the heart and the arrows expression around the yolk cell extension. (G) 24 hour, frontal view. gata5 expression in the heart on the left hand side of the embryo (arrowhead). More caudally, expression is across the midline in a thin layer of presumptive endoderm, and in the anterior lateral mesoderm (lm). (H) 30 hours (thick section). Cells expressing gata5 are close to the yolk cell (arrows). neural keel (nk); notochord (n); somites (s). At 4 days (10 mm sections) gata5 expression is maintained in the heart (arrow in I) and the gut (J). Labels in J are n, notochord; m, muscle; y, yolk. Scale bars, 250 μm (A-G); 50 μm (H); 25 μm (I,J).
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