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ZFIN ID: ZDB-FIG-140311-15
McCarroll et al., 2013 - Fgf3 and Fgf10a work in concert to promote maturation of the epibranchial placodes in zebrafish. PLoS One   8(12):e85087 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig. 3

Fgf10a and endodermally derived Fgf3 cooperate during EB placode formation.

(A-C) Confocal projections of Pax2a expression in control (A), casanova-MO injected (B), or casanova/fgf10a-MO coinjected (C) embryos at 24 hpf. (D-F) Confocal projections of 72 hpf TgBAC(phox2b:EGFP) in control (D), casanova-MO (E), or casanova/fgf10-MO (F) embryos. Note a complete loss of Pax2a and EGFP expression in EB placodes and ganglia, respectively, in casanova/fgf10a-MO embryos (C and F). Abbreviations: f, facial placode (A) or facial ganglia (D); ov, otic vesicle; g+v, glossopharyngeal/vagal placode; cas, casanova; g, glossopharyngeal ganglia; v1-v3, small vagal ganglia 1-3; v4, large vagal ganglion. Scale bars: 50 ┬Ám (A); 25 μm (D).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP w37Tg control Protruding-mouth facial ganglion IHC
Protruding-mouth glossopharyngeal ganglion IHC
Protruding-mouth vagal ganglion 1 IHC
Protruding-mouth vagal ganglion 2 IHC
Protruding-mouth vagal ganglion 3 IHC
Protruding-mouth vagal ganglion 4 IHC
w37Tg + MO1-sox32 standard conditions Protruding-mouth facial ganglion IHC
Protruding-mouth Not Detected glossopharyngeal ganglion IHC
Protruding-mouth Not Detected vagal ganglion 1 IHC
Protruding-mouth Not Detected vagal ganglion 2 IHC
Protruding-mouth Not Detected vagal ganglion 3 IHC
Protruding-mouth vagal ganglion 4 IHC
w37Tg + MO1-sox32 + MO3-fgf10a standard conditions Protruding-mouth Not Detected facial ganglion IHC
Protruding-mouth Not Detected glossopharyngeal ganglion IHC
Protruding-mouth Not Detected vagal ganglion 1 IHC
Protruding-mouth Not Detected vagal ganglion 2 IHC
Protruding-mouth Not Detected vagal ganglion 3 IHC
Protruding-mouth Not Detected vagal ganglion 4 IHC
pax2a Ab1-pax2 AB control Prim-5 facial placode IHC
Ab1-pax2 Prim-5 glossopharyngeal placode IHC
Ab1-pax2 Prim-5 otic vesicle IHC
Ab1-pax2 Prim-5 vagal placode 1 IHC
Ab1-pax2 Prim-5 vagal placode 2 IHC
Ab1-pax2 Prim-5 vagal placode 3 IHC
Ab1-pax2 Prim-5 vagal placode 4 IHC
Ab1-pax2 AB + MO1-sox32 standard conditions Prim-5 facial placode IHC
Ab1-pax2 Prim-5 glossopharyngeal placode IHC
Ab1-pax2 Prim-5 otic vesicle IHC
Ab1-pax2 Prim-5 vagal placode 1 IHC
Ab1-pax2 Prim-5 vagal placode 2 IHC
Ab1-pax2 Prim-5 vagal placode 3 IHC
Ab1-pax2 Prim-5 vagal placode 4 IHC
Ab1-pax2 AB + MO1-sox32 + MO3-fgf10a standard conditions Prim-5 Not Detected facial placode IHC
Ab1-pax2 Prim-5 Not Detected glossopharyngeal placode IHC
Ab1-pax2 Prim-5 otic vesicle IHC
Ab1-pax2 Prim-5 Not Detected vagal placode 1 IHC
Ab1-pax2 Prim-5 Not Detected vagal placode 2 IHC
Ab1-pax2 Prim-5 Not Detected vagal placode 3 IHC
Ab1-pax2 Prim-5 Not Detected vagal placode 4 IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-sox32 standard conditions Prim-5 ectodermal placode development decreased process quality, abnormal
Prim-5 glossopharyngeal placode poorly differentiated, abnormal
AB + MO1-sox32 + MO3-fgf10a standard conditions Prim-5 ectodermal placode development decreased process quality, abnormal
Prim-5 facial placode poorly differentiated, abnormal
Prim-5 glossopharyngeal placode poorly differentiated, abnormal
Prim-5 vagal placode 1 poorly differentiated, abnormal
Prim-5 vagal placode 2 poorly differentiated, abnormal
Prim-5 vagal placode 3 poorly differentiated, abnormal
Prim-5 vagal placode 4 poorly differentiated, abnormal
w37Tg + MO1-sox32 standard conditions Protruding-mouth cranial ganglion maturation decreased process quality, abnormal
Protruding-mouth glossopharyngeal ganglion poorly differentiated, abnormal
Protruding-mouth vagal ganglion 1 poorly differentiated, abnormal
Protruding-mouth vagal ganglion 2 poorly differentiated, abnormal
Protruding-mouth vagal ganglion 3 poorly differentiated, abnormal
w37Tg + MO1-sox32 + MO3-fgf10a standard conditions Protruding-mouth cranial ganglion maturation decreased process quality, abnormal
Protruding-mouth head lacks all parts of type facial ganglion, abnormal
Protruding-mouth head lacks all parts of type glossopharyngeal ganglion, abnormal
Protruding-mouth head lacks all parts of type vagal ganglion 1, abnormal
Protruding-mouth head lacks all parts of type vagal ganglion 2, abnormal
Protruding-mouth head lacks all parts of type vagal ganglion 3, abnormal
Protruding-mouth head lacks all parts of type vagal ganglion 4, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One