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ZFIN ID: ZDB-FIG-140311-14
McCarroll et al., 2013 - Fgf3 and Fgf10a work in concert to promote maturation of the epibranchial placodes in zebrafish. PLoS One   8(12):e85087 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig. 2

Fgf3 and Fgf10a are required for maturation of epibranchial placodes and development of the epibranchial ganglia.

(A-C) Confocal projections showing Pax2a expression in wild-type (A) fgf3+/- (B) and fgf3-/- (C) embryos at 24 hpf. (D-F) Confocal projections showing Pax2a expression in 24-hour old wild-type (D) fgf3+/- (E) and fgf3-/- (F) embryos injected with fgf10a-MO. Note the significant loss of Pax2a expression in the EB placodes (F; arrowheads). (G) Pax2a+ cell number in the facial, glossopharyngeal/vagal, and otic placodes for conditions in (A-F). Note the complete loss of Pax2a+ cells in the facial placode and a 4.5 fold reduction in the glossopharyngeal/vagal placode in fgf3-/-;fgf10a-MO embryos (ANOVA multiple comparison with Bonferroni’s correction; *P<0.05; ***P<<0.001; Error bars: standard error of mean; n=11 embryos per condition). (H-M) Confocal projections of 3 dpf wild-type (H), fgf3+/- (I) and fgf3-/- (J) embryos and wild-type (K), fgf3+/- (L) and fgf3-/- (M) embryos injected with fgf10a-MO. All embryos contain TgBAC(phox2b:EGFP) which marks EB ganglia. Note the loss of EGFP expression in the glossopharyngeal and three small vagal ganglia in fgf3-/- embryos (J) and the complete loss of EGFP expression in all EB ganglia in fgf3-/-;fgf10a-MO with the exception of a few EGFP+ cells in the region of the large vagal ganglion (M). Asterisk marks hindbrain neurons also expressing TgBAC(phox2b:EGFP). Abbreviations: f, facial placode (A) or facial ganglia (H); ov, otic vesicle; g+v, glossopharyngeal/vagal placode; g, glossopharyngeal ganglia; v1-v3, small vagal ganglia 1-3; v4, large vagal ganglion. Scale bars: 50 μm (A); 25 μm (H).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP fgf3t26212/+; w37Tg standard conditions Protruding-mouth facial ganglion IHC
Protruding-mouth glossopharyngeal ganglion IHC
Protruding-mouth vagal ganglion 1 IHC
Protruding-mouth vagal ganglion 2 IHC
Protruding-mouth vagal ganglion 3 IHC
Protruding-mouth vagal ganglion 4 IHC
fgf3t26212/+; w37Tg + MO3-fgf10a standard conditions Protruding-mouth facial ganglion IHC
Protruding-mouth glossopharyngeal ganglion IHC
Protruding-mouth vagal ganglion 1 IHC
Protruding-mouth vagal ganglion 2 IHC
Protruding-mouth vagal ganglion 3 IHC
Protruding-mouth vagal ganglion 4 IHC
fgf3t26212/t26212; w37Tg standard conditions Protruding-mouth facial ganglion IHC
Protruding-mouth Not Detected glossopharyngeal ganglion IHC
Protruding-mouth Not Detected vagal ganglion 1 IHC
Protruding-mouth Not Detected vagal ganglion 2 IHC
Protruding-mouth Not Detected vagal ganglion 3 IHC
Protruding-mouth vagal ganglion 4 IHC
fgf3t26212/t26212; w37Tg + MO3-fgf10a standard conditions Protruding-mouth Not Detected facial ganglion IHC
Protruding-mouth Not Detected glossopharyngeal ganglion IHC
Protruding-mouth Not Detected vagal ganglion 1 IHC
Protruding-mouth Not Detected vagal ganglion 2 IHC
Protruding-mouth Not Detected vagal ganglion 3 IHC
Protruding-mouth vagal ganglion 4 IHC
w37Tg standard conditions Protruding-mouth facial ganglion IHC
Protruding-mouth glossopharyngeal ganglion IHC
Protruding-mouth vagal ganglion 1 IHC
Protruding-mouth vagal ganglion 2 IHC
Protruding-mouth vagal ganglion 3 IHC
Protruding-mouth vagal ganglion 4 IHC
w37Tg + MO3-fgf10a standard conditions Protruding-mouth facial ganglion IHC
Protruding-mouth glossopharyngeal ganglion IHC
Protruding-mouth vagal ganglion 1 IHC
Protruding-mouth vagal ganglion 2 IHC
Protruding-mouth vagal ganglion 3 IHC
Protruding-mouth vagal ganglion 4 IHC
pax2a Ab1-pax2 AB standard conditions Prim-5 facial placode IHC
Ab1-pax2 Prim-5 glossopharyngeal placode IHC
Ab1-pax2 Prim-5 otic vesicle IHC
Ab1-pax2 Prim-5 vagal placode 1 IHC
Ab1-pax2 Prim-5 vagal placode 2 IHC
Ab1-pax2 Prim-5 vagal placode 3 IHC
Ab1-pax2 Prim-5 vagal placode 4 IHC
Ab1-pax2 AB + MO3-fgf10a standard conditions Prim-5 facial placode IHC
Ab1-pax2 Prim-5 glossopharyngeal placode IHC
Ab1-pax2 Prim-5 otic vesicle IHC
Ab1-pax2 Prim-5 vagal placode 1 IHC
Ab1-pax2 Prim-5 vagal placode 2 IHC
Ab1-pax2 Prim-5 vagal placode 3 IHC
Ab1-pax2 Prim-5 vagal placode 4 IHC
Ab1-pax2 fgf3t26212/+ standard conditions Prim-5 facial placode IHC
Ab1-pax2 Prim-5 glossopharyngeal placode IHC
Ab1-pax2 Prim-5 otic vesicle IHC
Ab1-pax2 Prim-5 vagal placode 1 IHC
Ab1-pax2 Prim-5 vagal placode 2 IHC
Ab1-pax2 Prim-5 vagal placode 3 IHC
Ab1-pax2 Prim-5 vagal placode 4 IHC
Ab1-pax2 fgf3t26212/+ + MO3-fgf10a standard conditions Prim-5 facial placode IHC
Ab1-pax2 Prim-5 glossopharyngeal placode IHC
Ab1-pax2 Prim-5 otic vesicle IHC
Ab1-pax2 Prim-5 vagal placode 1 IHC
Ab1-pax2 Prim-5 vagal placode 2 IHC
Ab1-pax2 Prim-5 vagal placode 3 IHC
Ab1-pax2 Prim-5 vagal placode 4 IHC
Ab1-pax2 fgf3t26212/t26212 standard conditions Prim-5 facial placode IHC
Ab1-pax2 Prim-5 glossopharyngeal placode IHC
Ab1-pax2 Prim-5 otic vesicle IHC
Ab1-pax2 Prim-5 vagal placode 1 IHC
Ab1-pax2 Prim-5 vagal placode 2 IHC
Ab1-pax2 Prim-5 vagal placode 3 IHC
Ab1-pax2 Prim-5 vagal placode 4 IHC
Ab1-pax2 fgf3t26212/t26212 + MO3-fgf10a standard conditions Prim-5 Not Detected facial placode IHC
Ab1-pax2 Prim-5 otic vesicle IHC
Ab1-pax2 Prim-5 vagal placode 1 IHC
Ab1-pax2 Prim-5 vagal placode 2 IHC
Ab1-pax2 Prim-5 vagal placode 3 IHC
Ab1-pax2 Prim-5 Not Detected vagal placode 4 IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO3-fgf10a standard conditions Prim-5 ectodermal placode development decreased process quality, abnormal
Prim-5 facial placode poorly differentiated, abnormal
Prim-5 glossopharyngeal placode poorly differentiated, abnormal
Prim-5 otic placode differentiated, normal
Prim-5 vagal placode 1 poorly differentiated, abnormal
Prim-5 vagal placode 2 poorly differentiated, abnormal
Prim-5 vagal placode 3 poorly differentiated, abnormal
Prim-5 vagal placode 4 poorly differentiated, abnormal
fgf3t26212/+ standard conditions Prim-5 otic placode differentiated, normal
fgf3t26212/+ + MO3-fgf10a standard conditions Prim-5 ectodermal placode development decreased process quality, abnormal
Prim-5 facial placode poorly differentiated, abnormal
Prim-5 glossopharyngeal placode poorly differentiated, abnormal
Prim-5 otic placode differentiated, normal
Prim-5 vagal placode 1 poorly differentiated, abnormal
Prim-5 vagal placode 2 poorly differentiated, abnormal
Prim-5 vagal placode 3 poorly differentiated, abnormal
Prim-5 vagal placode 4 poorly differentiated, abnormal
fgf3t26212/t26212 standard conditions Prim-5 ectodermal placode development decreased process quality, abnormal
Prim-5 facial placode poorly differentiated, abnormal
Prim-5 otic placode differentiated, normal
fgf3t26212/t26212 + MO3-fgf10a standard conditions Prim-5 ectodermal placode development decreased process quality, abnormal
Prim-5 glossopharyngeal placode poorly differentiated, abnormal
Prim-5 head lacks all parts of type facial placode, abnormal
Prim-5 otic placode differentiated, normal
Prim-5 vagal placode 1 poorly differentiated, abnormal
Prim-5 vagal placode 2 poorly differentiated, abnormal
Prim-5 vagal placode 3 poorly differentiated, abnormal
Prim-5 vagal placode 4 poorly differentiated, abnormal
fgf3t26212/t26212; w37Tg standard conditions Protruding-mouth ectodermal placode development decreased process quality, abnormal
Protruding-mouth head lacks all parts of type glossopharyngeal ganglion, abnormal
Protruding-mouth head lacks all parts of type vagal ganglion 1, abnormal
Protruding-mouth head lacks all parts of type vagal ganglion 2, abnormal
Protruding-mouth head lacks all parts of type vagal ganglion 3, abnormal
fgf3t26212/t26212; w37Tg + MO3-fgf10a standard conditions Protruding-mouth ectodermal placode development decreased process quality, abnormal
Protruding-mouth head lacks all parts of type facial ganglion, abnormal
Protruding-mouth head lacks all parts of type glossopharyngeal ganglion, abnormal
Protruding-mouth head lacks all parts of type vagal ganglion 1, abnormal
Protruding-mouth head lacks all parts of type vagal ganglion 2, abnormal
Protruding-mouth head lacks all parts of type vagal ganglion 3, abnormal
Protruding-mouth head lacks all parts of type vagal ganglion 4, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One