ZFIN ID: ZDB-FIG-140311-17
McCarroll et al., 2013 - Fgf3 and Fgf10a work in concert to promote maturation of the epibranchial placodes in zebrafish. PLoS One   8(12):e85087 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-25
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-25

Fig. 5

Fgf3 and Fgf10a are required for placode maturation and neurogenesis.

(A-F) Uninjected and fgf10a-MO injected progeny from fgf3+/-;TgBAC(neurog1-DSRed) crosses were collected at 36 hpf and immunolabeled for Pax2a and DSRed to visualize EB placodes and migrating neuroblasts, respectively. Wild-type (A) and fgf3+/- (B) panels shows neurog1:DSRed+ cells undergoing neurogenesis (magenta) at the dorsal aspect of the mature Pax2a+ EB placodes (green; dotted line). fgf3-/- embryos show a loss of properly formed Pax2a+ EB placodes in the region of the prospective glossopharyngeal and three small vagal ganglia and a concurrent loss of neurog1:DSRed+ cells in this region (C; bracket); however, the facial and large vagal placode/ganglia are still present (arrowheads). Analysis of fgf10a-MO injected wild-type (D) and fgf3+/- (E) embryos reveal ectopic neurogenesis as marked by neurog1:DSRed+ cells ventral to the posterior most aspect of the vagal placode (arrow). fgf3-/-;fgf10-MO embryos show a complete loss Pax2a expression in EB placodes and an absence of neurogenesis, except for a few neurog1:DSRed+ cells near the region of the large vagal ganglia (F; asterisk). Abbreviation: ngn:dsred, TgBAC(neurog1-DSRed); f, facial placode; g, glossopharyngeal placode; v, vagal placodes. Scale bar: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
DsRed fgf3t26212/+; nl6Tg standard conditions Prim-25 facial placode dorsal region IHC
Prim-25 glossopharyngeal placode dorsal region IHC
Prim-25 vagal placode 1 dorsal region IHC
Prim-25 vagal placode 2 dorsal region IHC
Prim-25 vagal placode 3 dorsal region IHC
Prim-25 vagal placode 4 dorsal region IHC
fgf3t26212/+; nl6Tg + MO3-fgf10a standard conditions Prim-25 vagal placode 4 neuroblast (sensu Vertebrata) IHC
Prim-25 vagal placode 4 ventral region IHC
fgf3t26212/t26212; nl6Tg standard conditions Prim-25 facial placode dorsal region IHC
Prim-25 Not Detected glossopharyngeal placode IHC
Prim-25 Not Detected vagal placode 1 IHC
Prim-25 Not Detected vagal placode 2 IHC
Prim-25 Not Detected vagal placode 3 IHC
Prim-25 vagal placode 4 dorsal region IHC
fgf3t26212/t26212; nl6Tg + MO3-fgf10a standard conditions Prim-25 Not Detected facial placode IHC
Prim-25 Not Detected glossopharyngeal placode IHC
Prim-25 Not Detected vagal placode 1 IHC
Prim-25 Not Detected vagal placode 2 IHC
Prim-25 Not Detected vagal placode 3 IHC
Prim-25 Not Detected vagal placode 4 IHC
nl6Tg standard conditions Prim-25 facial placode dorsal region IHC
Prim-25 glossopharyngeal placode dorsal region IHC
Prim-25 vagal placode 1 dorsal region IHC
Prim-25 vagal placode 2 dorsal region IHC
Prim-25 vagal placode 3 dorsal region IHC
Prim-25 vagal placode 4 dorsal region IHC
nl6Tg + MO3-fgf10a standard conditions Prim-25 vagal placode 4 neuroblast (sensu Vertebrata) IHC
Prim-25 vagal placode 4 ventral region IHC
pax2a Ab1-pax2 fgf3t26212/+; nl6Tg standard conditions Prim-25 facial placode IHC
Ab1-pax2 Prim-25 glossopharyngeal placode IHC
Ab1-pax2 Prim-25 vagal placode 1 IHC
Ab1-pax2 Prim-25 vagal placode 2 IHC
Ab1-pax2 Prim-25 vagal placode 3 IHC
Ab1-pax2 Prim-25 vagal placode 4 IHC
Ab1-pax2 fgf3t26212/+; nl6Tg + MO3-fgf10a standard conditions Prim-25 vagal placode 4 IHC
Ab1-pax2 fgf3t26212/t26212; nl6Tg standard conditions Prim-25 facial placode IHC
Ab1-pax2 Prim-25 Not Detected glossopharyngeal placode IHC
Ab1-pax2 Prim-25 otic vesicle IHC
Ab1-pax2 Prim-25 Not Detected vagal placode 1 IHC
Ab1-pax2 Prim-25 Not Detected vagal placode 2 IHC
Ab1-pax2 Prim-25 Not Detected vagal placode 3 IHC
Ab1-pax2 Prim-25 vagal placode 4 IHC
Ab1-pax2 fgf3t26212/t26212; nl6Tg + MO3-fgf10a standard conditions Prim-25 Not Detected facial placode IHC
Ab1-pax2 Prim-25 Not Detected glossopharyngeal placode IHC
Ab1-pax2 Prim-25 Not Detected vagal placode 1 IHC
Ab1-pax2 Prim-25 Not Detected vagal placode 2 IHC
Ab1-pax2 Prim-25 Not Detected vagal placode 3 IHC
Ab1-pax2 Prim-25 Not Detected vagal placode 4 IHC
Ab1-pax2 nl6Tg standard conditions Prim-25 facial placode IHC
Ab1-pax2 Prim-25 glossopharyngeal placode IHC
Ab1-pax2 Prim-25 otic vesicle IHC
Ab1-pax2 Prim-25 vagal placode 1 IHC
Ab1-pax2 Prim-25 vagal placode 2 IHC
Ab1-pax2 Prim-25 vagal placode 3 IHC
Ab1-pax2 Prim-25 vagal placode 4 IHC
Ab1-pax2 nl6Tg + MO3-fgf10a standard conditions Prim-25 facial placode IHC
Ab1-pax2 Prim-25 glossopharyngeal placode IHC
Ab1-pax2 Prim-25 vagal placode 1 IHC
Ab1-pax2 Prim-25 vagal placode 2 IHC
Ab1-pax2 Prim-25 vagal placode 3 IHC
Ab1-pax2 Prim-25 vagal placode 4 IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
fgf3t26212/+; nl6Tg + MO3-fgf10a standard conditions Prim-25 vagal placode 4 neuroblast (sensu Vertebrata) mislocalised ventrally, abnormal
fgf3t26212/t26212; nl6Tg standard conditions Prim-25 facial placode neurogenesis occurrence, normal
Prim-25 glossopharyngeal placode neurogenesis decreased occurrence, abnormal
Prim-25 vagal placode 1 neurogenesis decreased occurrence, abnormal
Prim-25 vagal placode 2 neurogenesis decreased occurrence, abnormal
Prim-25 vagal placode 3 neurogenesis decreased occurrence, abnormal
Prim-25 vagal placode 4 neurogenesis occurrence, normal
fgf3t26212/t26212; nl6Tg + MO3-fgf10a standard conditions Prim-25 facial placode neurogenesis decreased occurrence, abnormal
Prim-25 glossopharyngeal placode neurogenesis decreased occurrence, abnormal
Prim-25 vagal placode 1 neurogenesis decreased occurrence, abnormal
Prim-25 vagal placode 2 neurogenesis decreased occurrence, abnormal
Prim-25 vagal placode 3 neurogenesis decreased occurrence, abnormal
Prim-25 vagal placode 4 neurogenesis decreased occurrence, abnormal
nl6Tg + MO3-fgf10a standard conditions Prim-25 vagal placode 4 neuroblast (sensu Vertebrata) mislocalised ventrally, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One