FIGURE

Fig. 2

ID

ZDB-FIG-140303-22

Publication

Round et al., 2014 - Slitrk gene duplication and expression in the developing zebrafish nervous system

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Fig. 2

Seven slitrk genes are expressed in developing and adult zebrafish. Nonquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) was performed on total RNA isolated from whole zebrafish ranging in age from 12 hr postfertilization (hpf) to adulthood (90+ days postfertilization [dpf]). Transcripts corresponding to seven of eight predicted slitrks (1, 2, 3a, 3b, 4, 5a, 6) were detected from 48 dpf to adulthood, with slitrk6 detected as early as 24 hpf. Transcripts corresponding to slitrk5b were not detected at any age examined. To control for RNA integrity, β-actin was detected at all time-points examined (βactin+RT) and the absence of genomic DNA was confirmed by RT-PCR for β-actin in the absence of reverse transcriptase (βactin-RT).

Expression Data

Genes:	slitrk1 slitrk2 slitrk3a slitrk3b slitrk4 slitrk5a slitrk5b slitrk6
Fish:	WT
Anatomical Term:	whole organism
Stage Range:	Prim-5 to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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