ZFIN Image: Round et al., 2014, Fig. 2

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Figure Caption

Fig. 2

Seven slitrk genes are expressed in developing and adult zebrafish. Nonquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) was performed on total RNA isolated from whole zebrafish ranging in age from 12 hr postfertilization (hpf) to adulthood (90+ days postfertilization [dpf]). Transcripts corresponding to seven of eight predicted slitrks (1, 2, 3a, 3b, 4, 5a, 6) were detected from 48 dpf to adulthood, with slitrk6 detected as early as 24 hpf. Transcripts corresponding to slitrk5b were not detected at any age examined. To control for RNA integrity, β-actin was detected at all time-points examined (βactin+RT) and the absence of genomic DNA was confirmed by RT-PCR for β-actin in the absence of reverse transcriptase (βactin-RT).

Figure Data

Expression / Labeling details

Acknowledgments

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