FIGURE

Fig. 5

ID

ZDB-FIG-131218-65

Publication

Sarmah et al., 2013 - Complex cardiac defects after ethanol exposure during discrete cardiogenic events in zebrafish: Prevention with folic acid

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Fig. 5

Ethanol exposure during cardiac looping caused abnormal heart morphogenesis and defective endocardial cushions. A, G: Schematic diagram showing ethanol exposure timing. B: ISH detecting cmlc2 at 36 hpf: control, normal looping; EtOH-100 mM, modest looping; EtOH-150 mM, straight heart tube. C: Graph quantifies looped and straight heart at 36 hpf. D: 3D confocal images of Tg[cmlc2:GFP] embryos: control, normal heart chambers; ethanol-treated embryos, aberrant heart chambers. Yellow asterisks, AV canal. E: Ethanol-treated Tg[fli1:EGFP] embryos showed dispersed GFP and F-actin-positive cells (white dashed line) throughout the ventricle (compare control panel 2E). F: Alcam-stained Tg[cmlc2:GFP] embryo showed similar distribution of cardiomyocytes in the control and ethanol-treated embryos. G–I: Ethanol exposure during cardiac chamber growth did not lead to defective chamber wall formation. H: 3D confocal images of Tg[cmlc2:GFP] embryos. Control and E-100 mM, normal heart; E-150, nearly normal heart chamber. Yellow brackets, AV region. I: Alcam-stained Tg[cmlc2:GFP] embryos showed similar pattern of cardiomyocyte distribution in the ventricular wall in ethanol-treated and control embryos. White asterisk, alcam-positive hatching gland cells, which are outside the heart but seen in this 3D rendering. Representative cell shapes are highlighted in yellow. Anterior to the top.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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