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ZFIN ID: ZDB-FIG-130906-20
Armant et al., 2013 - Genome-wide, whole mount in situ analysis of transcriptional regulators in zebrafish embryos. Developmental Biology   380(2):351-62 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-5

Fig. 3

Assessment of TR gene expression by RNAseq. (A) Number of genes (white bars) and TRs (dashed bars) expressed at two different developmental stages and two adult body parts as determined by RNAseq. The total number of TR loci quantified by transcript counting over all stages is indicated as “Collapsed”. (B) Quantification of the total number of genes detected at 24 hpf by RNAseq in function of the sequencing depth. The number of detected genes is indicated as the mean from 3 biological replicates. (C) Level of expression of transcripts represented as FPKM from a selection of genes known to be expressed in the epiphysis (crx, otx5, nr2e3, aannt2), the retina (pax6b, hmx4) or tectum and retina (mycn and mych). The relative expression is indicated as the mean of FPKM from biological triplicates. (D) RNA in situ expression data of the selected transcripts at 24 hpf. *: epiphysis; arrow head: optic tectum.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
aanat2 AB standard conditions Prim-5 epiphysis ISH
crx AB standard conditions Prim-5 epiphysis ISH
hmx4 AB standard conditions Prim-5 brain ISH
Prim-5 optic cup ISH
mych AB standard conditions Prim-5 brain ISH
Prim-5 optic cup ISH
Prim-5 optic tectum ISH
mycn AB standard conditions Prim-5 brain ISH
Prim-5 optic cup ISH
Prim-5 optic tectum ISH
nr2e3 AB standard conditions Prim-5 epiphysis ISH
otx5 AB standard conditions Prim-5 epiphysis ISH
pax6b AB standard conditions Prim-5 brain ISH
Prim-5 optic cup ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 380(2), Armant, O., März, M., Schmidt, R., Ferg, M., Diotel, N., Ertzer, R., Bryne, J.C., Yang, L., Baader, I., Reischl, M., Legradi, J., Mikut, R., Stemple, D., Ijcken, W.V., van der Sloot, A., Lenhard, B., Strähle, U., and Rastegar, S., Genome-wide, whole mount in situ analysis of transcriptional regulators in zebrafish embryos, 351-62, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.