FIGURE

Fig. 2

ID

ZDB-FIG-130814-18

Publication

Ketley et al., 2013 - The miR-30 MicroRNA Family Targets smoothened to Regulate Hedgehog Signalling in Zebrafish Early Muscle Development

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Fig. 2

The miR-30 family is required during early embryonic development to regulate Hh pathway activity.

Embryo somite structure at 24 hpf is shown (A–D). Ptc1 expression analysis was used as a read out of Hh pathway activity, showing elevated levels in miR-30 morpholino and dnPKA treated embryos when compared to wild type embryos (E–H). Slow muscle fibre number was quantified by immunohistochemistry using the S58 antibody (yellow/green) and MF20 staining (red). Embryo sections are orientated dorsal side upwards (I–L). Images are shown of wild type embryos (A, E, I), miR-30 morpholino treated embryos (B, F, J), dnPKA treated embryos (C, G, K) and miR-30 overexpression embryos (D, H, L).

Expression Data

Gene:	ptch2
Antibodies:	Ab-MF20 Ab-S58
Fish:	WT WT + MO2-mir30e-2
Knockdown Reagent:	MO2-mir30e-2
Anatomical Terms:	muscle cell slow muscle cell whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-mir30e-2
Knockdown Reagent:	MO2-mir30e-2
Observed In:	slow muscle cell smoothened signaling pathway somite
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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