FIGURE

Fig. S4

ID

ZDB-FIG-130814-17

Publication

Ketley et al., 2013 - The miR-30 MicroRNA Family Targets smoothened to Regulate Hedgehog Signalling in Zebrafish Early Muscle Development

Other Figures

All Figure Page

Back to All Figure Page

Fig. S4

Cyclopamine treatment rescues the miR-30 morpholino phenotype. To achieve phenotypic rescue of the miR-30 morpholino phenotype cyclopamine was used at a concentration range of 100 μM-6.25 μM. At 6.25 μM the miR-30 morpholino phenotype improved to resemble the wild type phenotype with elongation of the tail and improved somite structure (F). Cyclopamine was dissolved in DMSO and both wild type and miR-30 morpholino injected embryos were treated with DMSO as a negative control (A–D) which had no effect on embryo development when compared to untreated. Wild type embryos treated with 6.25 μM cyclopamine showed a mild phenotype associated with Hh pathway inactivation with U shaped somites and a loss of brain chamber definition (E).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One