header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-130802-31
Lancman et al., 2013 - Specification of hepatopancreas progenitors in zebrafish by hnf1ba and wnt2bb. Development (Cambridge, England)   140(13):2669-2679 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Condition:
Anatomical Terms:
Stage Range: Prim-5 to Prim-25
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Prim-25

Fig. 5 Hnf1ba is required for Wnt signaling activity in the foregut endoderm. (A-C) Double antibody (myosin; blue) and in situ hybridization staining (ventral view) of 24 hpf wild-type foregut endoderm for expression of hnf1ba (A), wnt2bb (B) and prox1 (C, arrowheads). (D) Illustration summarizing foregut expression domains showing restricted prox1 expression within the anterior region of the hnf1ba foregut expression domain and adjacent to the wnt2bb expression domain in the lateral plate mesoderm. Pronephric hnf1ba expression (red asterisks in A) and dorsal pancreas prox1 expression (dp) are not depicted in the illustration. (E-G) Three-dimensional rendering (lateral view) of 36 hpf foregut endoderm in wild-type (E), Tg(hsp70l:wnt8a-GFP)w34 (F) and Tg(hsp70l:wnt8a-GFP)w34; hnf1bas430/hi2169 (G) embryos heat-shocked from 21-28 hpf at 37°C and stained for Prox1 and myosin. Compared with wild type, heat-shocked Tg(hsp70l:wnt8a-GFP)w34 embryos show significant posterior expansion of Prox1 in the foregut endoderm (yellow arrows; n=8/8). In Tg(hsp70l:wnt8a-GFP)w34; hnf1bas430/hi2169 embryos, only weak or no Prox1 expression is observed (yellow arrows; n=6/6). (H-L) Double antibody and in situ hybridization staining (ventral view) of cmyc at 36 hpf in wild-type (H), Tg(hsp70l:wnt8a-GFP)w34 (I), Tg(hsp70l:dkk1-GFP)w32 (J), hnf1bas430-/- (K) and Tg(hsp70l:dkk1-GFP)w32; hnf1bas430/hi216 (L) embryos heat-shocked from 21 to 28 hpf. cmyc expression is restricted in the wild-type foregut endoderm (white arrowhead in H) but is expanded posteriorly in Tg(hsp70l:wnt8a-GFP)w34 (yellow arrows in I; n=12/12) or severely reduced in Tg(hsp70l:dkk1-GFP)w32 (white arrowhead in J) embryos. cmyc expression is reduced or lost in the hnf1bas430 foregut endoderm (white arrowhead in K; n=4/4), and only weakly induced in Tg(hsp70l:wnt8a-GFP)w34; hnf1bas430/hi2169 embryos (yellow arrow in L; n=4/4).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
hnf1ba WT standard conditions Prim-5 intestinal bulb primordium ISH
Prim-5 pronephros ISH
myca WT heat shock Prim-25 intestinal bulb primordium ISH
hnf1bas430/+; hnf1bahi2169Tg/+; w34Tg heat shock Prim-25 intestinal bulb primordium ISH
hnf1bas430/s430 heat shock Prim-25 intestinal bulb primordium ISH
w32Tg heat shock Prim-25 intestinal bulb primordium ISH
w34Tg heat shock Prim-25 intestinal bulb primordium ISH
prox1a WT standard conditions Prim-5 intestinal bulb primordium ISH
Prim-5 posterior pancreatic bud ISH
wnt2bb WT standard conditions Prim-5 lateral plate mesoderm ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab3-prox1 IHC WT heat shock Prim-25 intestinal bulb primordium
IHC hnf1bas430/+; hnf1bahi2169Tg/+; w34Tg heat shock Prim-25 intestinal bulb primordium
IHC w34Tg heat shock Prim-25 intestinal bulb primordium
Phenotype Details
Fish Conditions Stage Phenotype
hnf1bas430/+; hnf1bahi2169Tg/+; w34Tg heat shock Prim-25 Wnt signaling pathway disrupted, abnormal
hnf1bas430/s430 heat shock Prim-25 Wnt signaling pathway disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development