ZFIN ID: ZDB-FIG-130607-10
Matsuda et al., 2013 - Lef1 regulates Dusp6 to influence neuromast formation and spacing in the zebrafish posterior lateral line primordium. Development (Cambridge, England)   140(11):2387-2397 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage Range: Prim-15 to Long-pec
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Long-pec

Fig. 4 Weakening Wnt signaling facilitates proto-neuromast maturation and deposition. (A-D) Wnt inhibitors reduce lef1 expression. lef1 expression is shown in the PLLp following exposure to DMSO control (A), IWR-1 (B) and XAV939 (C), and following heat shock-induced expression of ΔNTcf (D) at 31 hpf. (E,F) IWR-1 shrinks the broad leading fgf10 expression domain while exposing more spots of trailing fgf10 expression (arrows). (G,H) Premature appearance of atoh1b expression (arrows) in IWR-1-treated PLLp at ~31 hpf. (I,J) pea3 expression is not obviously altered in IWR-1-treated embryos. (K) Proto-neuromast formation is initiated closer to the leading edge in IWR-1-treated and BCI-treated embryos. **P<0.01, ***P<0.001. Error bars indicate s.d. (L) Comparison of the relative size of the lef1 expression domain at different stages of migration in embryos treated with DMSO (red) or 10 μM IWR-1 (blue), as in Fig. 2F,G. A simple linear regression model and a paired t-test confirmed that the length of the lef1 domain is significantly smaller in the IWR-1-treated embryos. P<0.0001. (M,N) Neuromast deposition in embryos treated with DMSO (M) or IWR-1 (N).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
atoh1b WT chemical treatment: dimethyl sulfoxide Prim-15 protoneuromast ISH
WT chemical treatment: pharmaceutical Prim-15 protoneuromast ISH
EGFP zf106Tg chemical treatment: dimethyl sulfoxide Long-pec neuromast IFL
zf106Tg chemical treatment: pharmaceutical Long-pec neuromast IFL
etv4 WT chemical treatment: dimethyl sulfoxide Prim-15 posterior lateral line primordium ISH
WT chemical treatment: pharmaceutical Prim-15 posterior lateral line primordium ISH
fgf10a WT chemical treatment: dimethyl sulfoxide Prim-15 posterior lateral line primordium ISH
WT chemical treatment: pharmaceutical Prim-15 posterior lateral line primordium ISH
lef1 WT chemical treatment: dimethyl sulfoxide Prim-15 posterior lateral line primordium ISH
WT chemical treatment: pharmaceutical Prim-15 posterior lateral line primordium ISH
WT chemical treatment: pharmaceutical Prim-15 posterior lateral line primordium ISH
w26Tg heat shock Prim-15 Not Detected posterior lateral line primordium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
zf106Tg chemical treatment: pharmaceutical Long-pec neuromast deposition process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development