FIGURE

Fig. 7

ID

ZDB-FIG-130508-2

Publication

Choudhry et al., 2013 - DiGeorge Syndrome Gene tbx1 Functions through wnt11r to Regulate Heart Looping and Differentiation

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Fig. 7

tbx1 and wnt11r regulate Alcama levels, and alcama regulates heart looping and differentiation.

(A, C) ISH analysis on 48 hpf embryos showing that wnt11r^-/- mutants have unaffected tbx1 expression. (B, D, E, F) Antibody staining showing that wnt11r^-/- (D) and tbx1^-/- (F) mutants have down-regulated Alcama expression when compared to WT siblings (B, E). cmlc2 ISH on 48 hpf embryos showing the various looping phenotypes observed in alcama morphants (H) and proper looping in an uninjected larva (G). notch1b is expressed in the AVC endocardium in an uninjected larva at 48 hpf (I), but is down-regulated in alcama morphants (J). Scale bars: 50 µm.

Expression Data

Genes:	alcama myl7 notch1b tbx1
Antibody:	zn-5
Fish:	WT wnt11^fh224/fh224 WT + MO3-alcama
Knockdown Reagent:	MO3-alcama
Anatomical Terms:	atrioventricular canal endocardium heart
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-alcama
Knockdown Reagent:	MO3-alcama
Observed In:	heart looping
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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