ZFIN ID: ZDB-FIG-130502-17
Song et al., 2013 - Pou5f1-dependent EGF expression controls e-cadherin endocytosis, cell adhesion, and zebrafish epiboly movements. Developmental Cell   24(5):486-501 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Shield
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 50%-epiboly to 75%-epiboly

Fig. 3 Inhibition of Rab5c Affects Epiboly(A–H) Live WT embryos injected with mRNA encoding membrane-tagged GFP (mGFP) (A and B), control morpholino (con Mo) (C and D), Rab5c MO (E and F), or RN-tre mRNA (G and H) are shown at stages corresponding to WT 50% epiboly and 80% epiboly. Lateral views, animal pole to the top. Scale bar, 50 μm.(I–P) E-cad subcellular localization in mgfp (I and J), control Mo (K and L), Rab5c Mo (M and N), or RN-tre mRNA (O and P) injected WT embryos at shield stage (confocal image of anti-E-cad whole mount immunofluorescence). Animal views. Scale bar, 10 μm.(Q and R) Quantification of (Q) relative DCL thickness at 50% epiboly and (R) epiboly progress at 50% epiboly and 80% epiboly stages (Error bars show SEM; p < 0.01, p < 0.001; n = 12 embryos each).See also Figure S3.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
AB1-cdh1 IHC AB/TL control Shield DEL endosome
IHC Shield DEL plasma membrane
IHC Shield EVL endosome
IHC Shield EVL plasma membrane
IHC AB/TL + MO1-rab5c standard conditions Shield DEL endosome
IHC Shield DEL plasma membrane
IHC Shield EVL endosome
IHC Shield EVL plasma membrane
Phenotype Details
Fish Conditions Stage Phenotype
AB/TL + MO1-rab5c standard conditions 50%-epiboly DEL increased thickness, abnormal
50%-epiboly - 75%-epiboly epiboly delayed, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 24(5), Song, S., Eckerle, S., Onichtchouk, D., Marrs, J.A., Nitschke, R., and Driever, W., Pou5f1-dependent EGF expression controls e-cadherin endocytosis, cell adhesion, and zebrafish epiboly movements, 486-501, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell