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ZFIN ID: ZDB-FIG-130412-15
Song et al., 2013 - Pou5f1-dependent EGF expression controls e-cadherin endocytosis, cell adhesion, and zebrafish epiboly movements. Developmental Cell   24(5):486-501 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Sphere to 75%-epiboly
PHENOTYPE:
Fish:
Observed In:
Stage: Shield

Fig. 1 Pou5f1 Controls Subcellular Localization of E-cad(A–D) Live WT and MZspg embryos at sphere and 70% epiboly stages. Arrowheads indicate vegetal front of blastoderm. Lateral views, animal pole to the top. Scale bar, 50 μm.(E–P) Confocal images of anti-E-cad ECD immunofluorescence whole-mount embryos reveal E-cad subcellular localization in WT and MZspg embryos; stages as indicated.(Q–R′) Confocal images of GFP-tagged E-cad expressed from injected mRNA. Live embryos were imaged at shield stage.(S) Quantification of the number of E-cad-positive intracellular vesicles at shield stage (n = 26 embryos each for WT and MZspg. Error bars show SEM; p < 0.001).(T–U3) Coimmunofluorescence of E-cad (red) and β-catenin (green) in WT and MZspg embryos at sphere stage in fixed whole mounts. Insets in (T)–(T′′′) show higher magnification.(V–W′) Confocal images ZO-1-EGFP signal expressed from injected mRNA (shield stage).(E–R′) and (T–W′) Animal views. Scale bars, 10 μm. See also Figure S1 and Movie S1.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
AB1-cdh1 IHC AB/TL standard conditions Sphere deep blastomere plasma membrane
IHC Sphere deep blastomere vesicle
IHC Sphere superficial blastomere endosome
IHC Sphere superficial blastomere plasma membrane
IHC Sphere superficial blastomere vesicle
IHC Shield DEL plasma membrane
IHC Shield DEL vesicle
IHC Shield EVL plasma membrane
IHC Shield EVL vesicle
IHC 75%-epiboly DEL plasma membrane
IHC 75%-epiboly DEL vesicle
IHC 75%-epiboly EVL plasma membrane
IHC 75%-epiboly EVL vesicle
IHC pou5f3m793/m793 standard conditions Sphere deep blastomere plasma membrane
IHC Sphere superficial blastomere plasma membrane
IHC Shield DEL plasma membrane
IHC Shield EVL plasma membrane
IHC 75%-epiboly DEL plasma membrane
IHC 75%-epiboly EVL plasma membrane
Ab1-ctnnb IHC AB/TL standard conditions Sphere superficial blastomere endosome
IHC Sphere superficial blastomere plasma membrane
IHC m1032Tg standard conditions Sphere superficial blastomere plasma membrane
Phenotype Details
Fish Conditions Stage Phenotype
pou5f3m793/m793 standard conditions Shield epiboly arrested, abnormal
Shield epiboly delayed, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 24(5), Song, S., Eckerle, S., Onichtchouk, D., Marrs, J.A., Nitschke, R., and Driever, W., Pou5f1-dependent EGF expression controls e-cadherin endocytosis, cell adhesion, and zebrafish epiboly movements, 486-501, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell