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MYCN reprogrammed hematopoiesis by regulating lineage-specific hematopoietic transcription factors. Scl (A) and lmo2 (C) expressed in WT, and Tg(MYCN:HSE:EGFP) F1, F2 generation embryos at 12 hpf, 18 hpf and 24 hpf. In situ hybridization of scl (B) and lmo2 (D) at 24 hpf. MYCN increased the two factors expression. Gata1 (E), pu.1 (G) and mpo (I) were detected by RQ-PCR in WT, Tg F1 and F2 embryos at 1 dpf, 3 dpf, 7 dpf and 60 dpf. In situ hybridization of gata1 (F), pu.1 (H) and mpo (J) in WT and Tg F2 embryos indicated that gata1 was downregulated, however, pu.1 and mpo were increased in Tg group, meaning that myelopoiesis was promoted while erythropoiesis was inhibited. Runx1 (K) and c-myb (L) expression were detected in WT and Tg F1, F2 generation zebrafish at 1 dpf, 3 dpf, 7 dpf and 60 dpf. Runx1, involved in definite hematopoiesis regulation, was upregulated in Tg group. C-myb, predominantly expressed in immature hematopoietic cells, were higher expression in Tg group, which suggests that MYCN overexpression results in accumulation of immature hematopoietic cells in adult fish. *, P<0.05; **, P<0.01
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