ZFIN ID: ZDB-FIG-130322-10
Clements et al., 2011 - A somitic Wnt16/Notch pathway specifies haematopoietic stem cells. Nature   474(7350):220-224 Full text @ Nature
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Day 4
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 1 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP la2Tg control Long-pec pronephric duct IFL
Long-pec ventral wall of dorsal aorta IFL
la2Tg + MO1-wnt16 + MO2-wnt16 standard conditions Long-pec pronephric duct IFL
la4Tg; zf169Tg control Long-pec ventral wall of dorsal aorta IFL
la4Tg; zf169Tg + MO1-wnt16 + MO2-wnt16 standard conditions Long-pec Not Detected ventral wall of dorsal aorta hematopoietic stem cell IFL
myb WT control Prim-15 ventral wall of dorsal aorta hematopoietic stem cell ISH
WT + MO1-wnt16 + MO2-wnt16 standard conditions Prim-15 Not Detected ventral wall of dorsal aorta hematopoietic stem cell ISH
rag1 WT control Day 4 thymus T cell ISH
WT + MO1-wnt16 + MO2-wnt16 standard conditions Day 4 Not Detected thymus T cell ISH
RFP la4Tg; zf169Tg control Long-pec ventral wall of dorsal aorta IFL
la4Tg; zf169Tg + MO1-wnt16 + MO2-wnt16 standard conditions Long-pec vasculature IFL
runx1 WT control Prim-5 neuron ISH
Prim-5 ventral wall of dorsal aorta hematopoietic stem cell ISH
WT + MO1-wnt16 + MO2-wnt16 standard conditions Prim-5 neuron ISH
Prim-5 Not Detected ventral wall of dorsal aorta hematopoietic stem cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-wnt16 + MO2-wnt16 standard conditions Prim-5 hematopoietic stem cell absent, abnormal
Prim-5 hematopoietic stem cell differentiation disrupted, abnormal
Prim-5 thymus epithelium present, normal
Prim-5 thymus T cell absent, abnormal
Full text @ Nature