FIGURE

Fig. 5

ID
ZDB-FIG-130306-12
Publication
Espina et al., 2013 - csrnp1a Is Necessary for the Development of Primitive Hematopoiesis Progenitors in Zebrafish
Other Figures
(all 9)
All Figure Page
Back to All Figure Page
Fig. 5

Erythroid/Gata1 precursors are affected by csrnp1a knockdown.

(A, C, E) Control and (B, D, F) morphant embryos. At 8 somite stage the expression of the erythroid marker klf4 was not affected in morphant embryos (A, B) (100%, n = 48). However a clear decrease in gata1 expression was detected in csrnp1a deficient embryos (compare C with D) (54,8%; n = 23/42) without major changes in the number of myoD stripes (7,1%; n = 3/42, more than 2 stripes), which served as readout of developmental progression. Control embryos showed normal gata1 expression in 100% of the cases (n = 0/27) and 3,7% have altered myoD expression (n = 1/27). In agreement with gata1 inhibition, at 32 hpf terminal differentiation marker, hbae1, was reduced in morphant embryos (E, F) (32,6%; n = 14/43).
Expression Data
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 5-9 somites to Prim-15

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 5-9 somites to Prim-15

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.