Fig. 6
- ID
- ZDB-FIG-130306-13
- Publication
- Espina et al., 2013 - csrnp1a Is Necessary for the Development of Primitive Hematopoiesis Progenitors in Zebrafish
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Myeloid lineage is affected by csrnp1a knockdown. Morphants embryos show an alteration in the spi1 signal (A to D; A, B lateral views anterior lo left; and D, E anterior views). 85,7% of control embryos exhibit normal spi1 expression pattern (n = 42/49) (A, C), while csrnp1a morphants exhibit altered spi1 expression in 59,2% (n = 61/103) (B, D). The spi1 changes are accompanied by a significant decrease in proliferation (showed as the number of H3P within the spi1 signal area) (E, F, and G). Finally, a decrease in the macrophage specific marker lcp1 (43,8%; n = 14/32) is evidenced by ISH (H, I), and a clear reduction in the neutrophil specific marker mpo is evident in the transgenic line expressing GFP under the mpo promoter Tg(Bacmpo:GFP)i114 (J, K)(47,2%; n = 25/53). All are lateral views, anterior to the left. |
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Stage Range: | 10-13 somites to Prim-15 |
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Knockdown Reagent: | |
Observed In: | |
Stage Range: | 10-13 somites to Prim-15 |