ZFIN ID: ZDB-FIG-130220-26
Henriques et al., 2013 - Telomerase is required for zebrafish lifespan. PLoS Genetics   9(1):e1003214 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Days 30-44 to Adult
PHENOTYPE:
Fish:
Observed In:
Stage Range: Days 30-44 to Adult

Fig. 5 Tissue degeneration is accompanied by p53 induction with a puma acute response and sustained increase of cyclin G1 and cdkn1a expression.

A) Immunoblot analysis of p53 in 10-month old WT and tert-/- testes and gut lysates. 6 month-old WT zebrafish were injected with the DNA damaging agent doxorubicin to serve as positive control for p53 activation. Asterisk depicts a non-specific cross-reactive band that serves as loading control. RT-qPCR analysis showing expression of B) pro-apoptotic (puma) and cell cycle arrest targets (C) cyclin G1 and D) cdkn1a) in testes, head kidney, gut and muscle of 1, 3, 6 and c.12 months old WT and tert-/- zebrafish (N = 3 to 8 fish per genotype). Data are represented as mean +/- SEM. Rel. mRNA refers to relative mRNA levels of each gene normalized to beta-actin.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
bbc3 WT standard conditions Days 30-44 male organism trunk musculature RTPCR
Adult male organism gut RTPCR
Adult male organism head kidney RTPCR
Adult male organism trunk musculature RTPCR
Adult testis RTPCR
terthu3430/hu3430(AB) standard conditions Days 30-44 male organism trunk musculature RTPCR
Adult male organism gut RTPCR
Adult male organism head kidney RTPCR
Adult male organism trunk musculature RTPCR
Adult testis RTPCR
ccng1 WT standard conditions Days 30-44 male organism gut RTPCR
Days 30-44 male organism head kidney RTPCR
Days 30-44 male organism trunk musculature RTPCR
Days 30-44 testis RTPCR
Adult male organism gut RTPCR
Adult male organism head kidney RTPCR
Adult male organism trunk musculature RTPCR
Adult testis RTPCR
terthu3430/hu3430(AB) standard conditions Days 30-44 male organism gut RTPCR
Days 30-44 male organism head kidney RTPCR
Days 30-44 male organism trunk musculature RTPCR
Days 30-44 testis RTPCR
Adult male organism gut RTPCR
Adult male organism head kidney RTPCR
Adult male organism trunk musculature RTPCR
Adult testis RTPCR
cdkn1a WT standard conditions Days 30-44 male organism gut RTPCR
Days 30-44 male organism head kidney RTPCR
Days 30-44 male organism trunk musculature RTPCR
Adult male organism gut RTPCR
Adult male organism head kidney RTPCR
Adult male organism trunk musculature RTPCR
Adult testis RTPCR
terthu3430/hu3430(AB) standard conditions Days 30-44 male organism gut RTPCR
Days 30-44 male organism head kidney RTPCR
Days 30-44 male organism trunk musculature RTPCR
Adult male organism gut RTPCR
Adult male organism head kidney RTPCR
Adult male organism trunk musculature RTPCR
Adult testis RTPCR
tp53 Ab7-tp53 WT standard conditions Adult Not Detected male organism gut WB
Ab7-tp53 Adult Not Detected testis WB
Ab7-tp53 terthu3430/hu3430(AB) standard conditions Adult male organism gut WB
Ab7-tp53 Adult testis WB
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
terthu3430/hu3430(AB) standard conditions Days 30-44 regulation of cell cycle increased occurrence, abnormal
Days 30-44 regulation of signal transduction by p53 class mediator increased process quality, abnormal
Adult regulation of cell cycle increased occurrence, abnormal
Adult regulation of signal transduction by p53 class mediator increased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.