IMAGE

Fig. 5

ID

ZDB-IMAGE-130220-24

Genes

Antibodies

Ab7-tp53

Source

Figures for Henriques et al., 2013

Image

Figure Caption

Fig. 5 Tissue degeneration is accompanied by p53 induction with a puma acute response and sustained increase of cyclin G1 and cdkn1a expression.

A) Immunoblot analysis of p53 in 10-month old WT and tert^-/- testes and gut lysates. 6 month-old WT zebrafish were injected with the DNA damaging agent doxorubicin to serve as positive control for p53 activation. Asterisk depicts a non-specific cross-reactive band that serves as loading control. RT-qPCR analysis showing expression of B) pro-apoptotic (puma) and cell cycle arrest targets (C) cyclin G1 and D) cdkn1a) in testes, head kidney, gut and muscle of 1, 3, 6 and c.12 months old WT and tert^-/- zebrafish (N = 3 to 8 fish per genotype). Data are represented as mean +/- SEM. Rel. mRNA refers to relative mRNA levels of each gene normalized to beta-actin.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.