FIGURE

Fig. 5

ID

ZDB-FIG-130110-25

Publication

Dalcq et al., 2012 - RUNX3, EGR1 and SOX9B Form a Regulatory Cascade Required to Modulate BMP-Signaling during Cranial Cartilage Development in Zebrafish

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Fig. 5

Runx3 is required for pharyngeal egr1 and sox9b expression at 48 hpf.

Lateral views of in situ hybridizations (A,B,E-L) with indicated markers and ventral views of Alcian Blue stained embryos (C,D), anterior to the left. Scale bars 100 μm. (A,B) Endodermal runx3 expression in the pharyngeal region is not altered in 4 ng MOegr1 spl morphants. (C,D) runx3 knock-down using 2 ng MOrunx3 tr leads to total absence of viscerocranium and the anterior neurocranium (D) compared to control (C) embryos. (E,F) runx3 morphants do not express runx2b in pharyngeal cartilage precursor cells. (G,H) runx3 morphants do not express egr1 transcripts in pharyngeal endoderm. (I,J) The endodermal marker sox9b is absent in pharyngeal endoderm when runx3 expression is blocked. (K,L) runx3 knock-down does not affect expression of pharyngeal endodermal marker nkx2.3 at 48 hpf. Trigeminal ganglia (tg), pharyngeal endoderm (pe), cleithrum (cl), Meckel’s cartilage (m), palatoquadrate (pq), hyosymplectic (hs), ceratohyal (hs), ceratobranchials 1 to 5 (cb1-5), ethmoid plate (ep), otic vesivle (ov).

Expression Data

Genes:	egr1 nkx2.3 runx2b runx3 sox9b
Fish:	WT WT + MO1-runx3 WT + MO3-egr1
Knockdown Reagents:	MO1-runx3 MO3-egr1
Anatomical Terms:	brain ceratobranchial cartilage ceratohyal cartilage cleithrum ethmoid cartilage hyosymplectic cartilage pharyngeal arch 1 pharyngeal pouch telencephalon trigeminal ganglion
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-runx3
Knockdown Reagent:	MO1-runx3
Observed In:	ceratobranchial cartilage ceratohyal cartilage hyosymplectic cartilage Meckel's cartilage neurocranium anterior region palatoquadrate cartilage splanchnocranium
Stage:	Day 4

Phenotype Detail

Acknowledgments

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