FIGURE

Fig. 3

ID

ZDB-FIG-130109-17

Publication

Clément et al., 2012 - Functional redundancy between Cdc14 phosphatases in zebrafish ciliogenesis

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Fig. 3

Effect of Cdc14A1 loss of function in LR asymmetry establishment. ISH and expression pattern quantification in non-injected, cdc14a1 and cdc14b MO- injected embryos. A-D: nkx2.5 expression at 31 hpf. E–H: foxa3 expression at 50 hpf. I-L: spaw expression at 19 hpf. Scale bar = 100 μm. M: Rescue of LR asymmetry defect in Cdc14A1-depleted embryos. Heart tube shifting was determined at 31 hpf. Numbers of embryos analyzed are indicated. Data are represented as mean ± SEM. Statistics were done using Student′s t-Test (D,H,L) and are only presented for the left-sided heart tube class in M.

Expression Data

Genes:	foxa3 nkx2.5 spaw
Fish:	AB AB + MO1-cdc14aa AB + MO1-cdc14b
Knockdown Reagents:	MO1-cdc14aa MO1-cdc14b
Anatomical Terms:	gut heart tube lateral plate mesoderm left side lateral plate mesoderm right side liver pancreas
Stage Range:	20-25 somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-cdc14aa AB + MO1-cdc14b AB + MO2-cdc14aa
Knockdown Reagents:	MO1-cdc14aa MO1-cdc14b MO2-cdc14aa
Observed In:	embryonic heart tube left/right pattern formation heart jogging left/right pattern formation
Stage Range:	20-25 somites to Long-pec

Phenotype Detail

Acknowledgments

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