ZFIN ID: ZDB-FIG-121205-6
Blaker-Lee et al., 2012 - Zebrafish homologs of 16p11.2, a genomic region associated with brain disorders, are active during brain development, and include two deletion dosage sensor genes. Disease models & mechanisms   5(6):834-851 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 2 Loss of function (LOF) embryos have abnormal brain and tail morphology. (A) Embryonic phenotypes were observed at 24 hpf, after LOF was caused by injection of antisense MOs (supplementary material Table S2) at the one- to two-cell stage (supplementary material Table S3). Genes assayed (supplementary material Table S1) are indicated above each set of images. ‘Control’ embryos were injected with control MO (see Methods). Brain ventricles were injected with Texas Red dextran, and bright-field and fluorescence images superimposed. Images are representative of the phenotypes observed in at least 70% of embryos, over two to seven independent experiments, with 50–350 embryos assayed in total per gene (supplementary material Table S3). (Aa-Aw) Dorsal views; (Aa′-Aw′) lateral close-up; (Aa′′-Aw′′) full-embryo lateral view. (Aa-Aa′′) Schematics of embryo landmarks. F, forebrain ventricle; M, midbrain ventricle; H, hindbrain ventricle. (Ab-Aw, Ab′-Aw′) Anterior to the left, and images are shown at equivalent magnification. (B) Phenotypic group for which LOF embryos have narrow midbrain and hindbrain ventricles. The gene assayed is indicated above each panel. Dorsal views, anterior to the left. F, forebrain ventricle; M, midbrain ventricle; H, hindbrain ventricle; MHB, midbrain-hindbrain boundary. (C) Phenotypic group for which LOF embryos have a straight midbrain. The gene assayed is indicated above each panel. Dorsal views, anterior to the left. FB, forebrain; MHB, midbrain-hindbrain boundary; asterisk, midbrain hingepoint. Scale bars: 150 μm. Embryo images in B and C are the dorsal view of panels of A except for the control embryo and the gdpd3 LOF embryo in C.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-aldoaa standard conditions Prim-5 brain morphology, abnormal
Prim-5 forebrain decreased width, abnormal
Prim-5 midbrain shape, abnormal
WT + MO1-asphd1 standard conditions Prim-5 brain morphology, abnormal
WT + MO1-coro1a standard conditions Prim-5 brain morphology, abnormal
WT + MO1-doc2a standard conditions Prim-5 brain morphology, abnormal
Prim-5 ventricular system decreased size, abnormal
WT + MO1-fam57ba standard conditions Prim-5 brain morphology, abnormal
Prim-5 forebrain decreased width, abnormal
Prim-5 midbrain shape, abnormal
WT + MO1-gdpd3a standard conditions Prim-5 brain morphology, abnormal
Prim-5 midbrain shape, abnormal
WT + MO1-hirip3 standard conditions Prim-5 brain morphology, abnormal
Prim-5 ventricular system decreased size, abnormal
WT + MO1-ino80e standard conditions Prim-5 brain morphology, abnormal
WT + MO1-kif22 standard conditions Prim-5 brain morphology, abnormal
WT + MO1-maza standard conditions Prim-5 brain morphology, abnormal
Prim-5 forebrain decreased width, abnormal
Prim-5 midbrain shape, abnormal
WT + MO1-pagr1 standard conditions Prim-5 brain morphology, abnormal
Prim-5 ventricular system decreased size, abnormal
WT + MO1-taok2a standard conditions Prim-5 brain morphology, abnormal
WT + MO1-taok2b standard conditions Prim-5 brain morphology, abnormal
Prim-5 ventricular system decreased size, abnormal
WT + MO1-ypel3 standard conditions Prim-5 brain morphology, abnormal
Prim-5 midbrain shape, abnormal
WT + MO2-kctd13 standard conditions Prim-5 brain morphology, abnormal
Prim-5 ventricular system decreased size, abnormal
WT + MO2-sez6l2 standard conditions Prim-5 brain morphology, abnormal
WT + MO3-cdipt standard conditions Prim-5 brain morphology, abnormal
Prim-5 ventricular system decreased size, abnormal
WT + MO3-mvp standard conditions Prim-5 brain morphology, abnormal
WT + MO5-mapk3 standard conditions Prim-5 brain morphology, abnormal
WT + MO5-ppp4ca standard conditions Prim-5 brain morphology, abnormal
Prim-5 midbrain shape, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.