Loss of function (LOF) embryos have abnormal brain and tail morphology. (A) Embryonic phenotypes were observed at 24 hpf, after LOF was caused by injection of antisense MOs (supplementary material Table S2) at the one- to two-cell stage (supplementary material Table S3). Genes assayed (supplementary material Table S1) are indicated above each set of images. ‘Control’ embryos were injected with control MO (see Methods). Brain ventricles were injected with Texas Red dextran, and bright-field and fluorescence images superimposed. Images are representative of the phenotypes observed in at least 70% of embryos, over two to seven independent experiments, with 50–350 embryos assayed in total per gene (supplementary material Table S3). (Aa-Aw) Dorsal views; (Aa′-Aw′) lateral close-up; (Aa′′-Aw′′) full-embryo lateral view. (Aa-Aa′′) Schematics of embryo landmarks. F, forebrain ventricle; M, midbrain ventricle; H, hindbrain ventricle. (Ab-Aw, Ab′-Aw′) Anterior to the left, and images are shown at equivalent magnification. (B) Phenotypic group for which LOF embryos have narrow midbrain and hindbrain ventricles. The gene assayed is indicated above each panel. Dorsal views, anterior to the left. F, forebrain ventricle; M, midbrain ventricle; H, hindbrain ventricle; MHB, midbrain-hindbrain boundary. (C) Phenotypic group for which LOF embryos have a straight midbrain. The gene assayed is indicated above each panel. Dorsal views, anterior to the left. FB, forebrain; MHB, midbrain-hindbrain boundary; asterisk, midbrain hingepoint. Scale bars: 150 μm. Embryo images in B and C are the dorsal view of panels of A except for the control embryo and the gdpd3 LOF embryo in C.