FIGURE

Fig. 4

ID

ZDB-FIG-120517-4

Publication

Gongal et al., 2011 - Hmx4 regulates Sonic hedgehog signaling through control of retinoic acid synthesis during forebrain patterning

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Fig. 4

hmx4 morphants have disrupted RA metabolism gene expression. aldh1a2 is reduced in the paraxial mesoderm of hmx4 morphants at 90% epiboly (A), and in the somites (B) and dorsal optic vesicle (C) at 18 hpf. (D) qPCR showing the significantly reduced levels of aldh1a2 transcription in hmx4 morphants (see text for statistical tests), with the levels in wild type embryos set to 1. Error bars indicate standard deviation. (E) cyp26a1 is mildly expanded towards the posterior in hmx4 morphants, and abolished in the presumptive hindbrain domain (bracket). In hmx4 morphants, the r6-7 domain of cyp26b1 is abolished (F), while the r3-r4 domain of cyp26c1 is not affected (G).

Expression Data

Genes:	aldh1a2 cyp26a1 cyp26b1 cyp26c1
Fish:	AB AB + MO1-hmx4 + MO2-hmx4 + MO4-tp53
Knockdown Reagents:	MO1-hmx4 MO2-hmx4 MO4-tp53
Anatomical Terms:	hindbrain neural plate margin neuroectoderm optic vesicle presumptive paraxial mesoderm presumptive rhombomere 3 presumptive rhombomere 4 presumptive rhombomere 6 presumptive rhombomere 7 somite
Stage Range:	90%-epiboly to 14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 355(1), Gongal, P.A., March, L.D., Holly, V.L., Pillay, L.M., Berry-Wynne, K.M., Kagechika, H., and Waskiewicz, A.J., Hmx4 regulates Sonic hedgehog signaling through control of retinoic acid synthesis during forebrain patterning, 55-64, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.