FIGURE

Fig. S4

ID

ZDB-FIG-120412-39

Publication

Camp et al., 2012 - Intronic Cis-Regulatory Modules Mediate Tissue-Specific and Microbial Control of angptl4/fiaf Transcription

Other Figures

All Figure Page

Back to All Figure Page

Fig. S4

The zebrafish angptl4 in3.4 intestinal module exhibits hallmarks of a classical enhancer. (A) Dr in3.4 was cloned in an inverted orientation (in3.4(ds-iv)) downstream of GFP driven by -1 kb of the angptl4 promoter (Tg(-1angptl4:GFP:in3.4inv)). Mosaic and stable intestinal expression patterns are indistinguishable from those when in3.4 is upstream of the Fos minimal promoter (see Figure 3). The white arrow marks the boundary between the anterior intestine (segment 1) and mid-intestine (segment 2). The marked liver expression is likely conferred by the -1 kb angptl4 promoter (see Figure S3F). (B) The in3.2 module drives expression of a reporter (tdTomato) in the intestinal epithelium of adult zebrafish. (C) Nuclei were isolated from adult zebrafish epithelial cells and subjected to increasing concentrations of DNase I. Digested DNA from 0.5 units DNase I was used for quantitative PCR shown in Figure 3P.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.