ZFIN ID: ZDB-FIG-111018-18
Mouillesseaux et al., 2011 - Mutation in utp15 Disrupts Vascular Patterning in a p53-Dependent Manner in Zebrafish Embryos. PLoS One   6(9):e25013 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 1 Confocal microscopic analysis of angiogenesis in LA1908 embryos.

A–F, Confocal z-stack projection images were captured of the trunk and tail vasculature of wild type (A, C, E) and LA1908 mutant (B, D, F) embryos at 25 (A, B), 30 (C, D), or 48 hpf (E, F). CVP endothelial tip cells are indicated by arrowheads; extent of ISV maturation is indicated by arrows in A–D. Delay of primary ISV sprouting is most prominent at 30 hpf (C and D, arrows), when WT ISVs have reached their dorsal terminus and branched to form the DLAV (C), while mutant ISVs remain stunted (D). By 48 hpf, ISV number is equivalent between WT and mutant embryos and DLAV has formed in both groups (E, F, arrows). Defects in CVP angiogenesis result in a thinner and shorter plexus that lacks the complexity of WT embryos (F versus E). Insets in E and F, enlarged regions indicated by yellow rectangles, showing ISV lumens. Scale bars are 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
GFP la116Tg standard conditions Prim-5 caudal vein plexus endothelial tip cell IFL
Prim-5 intersegmental vessel IFL
Prim-5 post-vent vasculature IFL
Prim-5 trunk vasculature IFL
Prim-15 caudal vein plexus endothelial tip cell IFL
Prim-15 intersegmental vessel IFL
Prim-15 posterior cardinal vein IFL
Prim-15 trunk vasculature IFL
Long-pec caudal vein plexus IFL
Long-pec dorsal longitudinal anastomotic vessel IFL
Long-pec intersegmental vessel IFL
Long-pec posterior cardinal vein IFL
Long-pec trunk vasculature IFL
utp15la1908/la1908; la116Tg standard conditions Prim-5 Not Detected caudal vein plexus endothelial tip cell IFL
Prim-5 intersegmental vessel IFL
Prim-5 post-vent vasculature IFL
Prim-5 trunk vasculature IFL
Prim-15 caudal vein plexus endothelial tip cell IFL
Prim-15 intersegmental vessel IFL
Prim-15 posterior cardinal vein IFL
Prim-15 trunk vasculature IFL
Long-pec caudal vein plexus IFL
Long-pec dorsal longitudinal anastomotic vessel IFL
Long-pec intersegmental vessel IFL
Long-pec posterior cardinal vein IFL
Long-pec trunk vasculature IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
utp15la1908/la1908; la116Tg standard conditions Prim-5 caudal vein plexus endothelial tip cell absent, abnormal
Prim-5 intersegmental vessel decreased amount, abnormal
Prim-5 intersegmental vessel decreased length, abnormal
Prim-5 intersegmental vessel hypoplastic, abnormal
Prim-5 sprouting angiogenesis delayed, abnormal
Prim-15 caudal vein plexus endothelial tip cell decreased amount, abnormal
Prim-15 dorsal longitudinal anastomotic vessel aplastic, abnormal
Prim-15 intersegmental vessel decreased length, abnormal
Prim-15 intersegmental vessel hypoplastic, abnormal
Prim-15 sprouting angiogenesis delayed, abnormal
Long-pec angiogenesis delayed, abnormal
Long-pec caudal vein plexus complexity, abnormal
Long-pec caudal vein plexus decreased length, abnormal
Long-pec caudal vein plexus decreased thickness, abnormal
Long-pec intersegmental vessel decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One