ZFIN ID: ZDB-FIG-111018-20
Mouillesseaux et al., 2011 - Mutation in utp15 Disrupts Vascular Patterning in a p53-Dependent Manner in Zebrafish Embryos. PLoS One   6(9):e25013 Full text @ PLoS One
Anatomical Terms:
Stage: Prim-5
Observed In:
Stage: Prim-5

Fig. 3 Apoptosis is pervasive in neural tissues of LA1908 mutant embryos.

A–B, Brightfield phase-contrast imaging of mutant embryos revealed craniofacial disorganization and extensive cell death (hazy dark brown appearance in midbrain in B versus A). C–D′, Apoptosis is significantly induced in LA1908 mutant embryos (D, D′) relative to stage-matched WT siblings (C, C′). Green fluorescence marks the endothelial cells. Red fluorescence indicates apoptotic cells detected by TUNEL assay. ACeV, Anterior cerebral vein. ey, eye primordium. hrt, embryonic heart. MCeV, Middle cerebral vein. PMBC, Primordial midbrain channel. E, Quantitative and statistical analyses of apoptotic bodies at several stages of development in WT versus mutant embryos. * = p-value<0.05, ** = p-value<0.005, *** = p-value<0.00005 by two-tailed t-test, n = 3 embryos for each condition, data are average±SEM.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP la116Tg standard conditions Prim-5 anterior cerebral vein IFL
Prim-5 cranial blood vessel blood vessel endothelial cell IFL
Prim-5 mid cerebral vein IFL
Prim-5 primordial midbrain channel IFL
utp15la1908/la1908; la116Tg standard conditions Prim-5 cranial blood vessel blood vessel endothelial cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
utp15la1908/la1908; la116Tg standard conditions Prim-5 eye decreased size, abnormal
Prim-5 head decreased size, abnormal
Prim-5 midbrain apoptotic, abnormal
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One