FIGURE

Fig. 4

ID

ZDB-FIG-111017-8

Publication

Ramachandran et al., 2011 - Ascl1a/Dkk/ beta -catenin signaling pathway is necessary and glycogen synthase kinase-3 beta inhibition is sufficient for zebrafish retina regeneration

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Fig. 4

β-Catenin stabilization stimulates MG dedifferentiation and the generation of multiple retinal cell types in the uninjured retina. (A) Intravitreal injection of GSK-3β inhibitor induces GFP, β-catenin, and cell proliferation in the uninjured retina of 1016 tuba1a:gfp transgenic fish. (B and C) Expression of regeneration-associated genes in uninjured retinas treated with the GSK-3β inhibitor. *P < 0.0001. (D) GSK-3β inhibitor-induced retinal progenitors proliferate and generate all major retinal cell types. (E and F) Effect of Ascl1a knockdown on MG proliferation in the GSK-3β inhibitor-treated retina. *P < 0.002. (G) Effect of Ascl1a knockdown on genes associated with MG dedifferentiation in the GSK-3β inhibitor-treated retina. [Scale bars, 10 μm (A and D); 20 μm (E).] Abbreviations are as in Fig. 1.

Expression Data

Genes:	ascl1a fzd2 GFP lin28ab myca pax6a wnt4
Antibodies:	Ab1-elavl Ab1-glul Ab2-ctnnb zn-5 zpr-1
Fish:	mi4Tg nt11Tg mi4Tg + MO1-ascl1a
Conditions:	chemical treatment: pharmaceutical physical alteration: anatomical structure
Knockdown Reagent:	MO1-ascl1a
Anatomical Terms:	amacrine cell Muller cell retina retinal ganglion cell retinal ganglion cell layer retinal inner nuclear layer retinal outer nuclear layer visible light photoreceptor cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA