Fig. 1

Characterisation of the zebrafish lef1^u767 mutant. (A-C) Lateral views of wild-type (A), lef1^u767 (B) and lef1 morphant (C) 3 dpf zebrafish embryos stained with DiAsp/DiOC₆. Arrows indicate positions of PLL neuromasts. (D) RT-PCR from sibling and lef1^u767 cDNA using primers targeting exons 1 and 3. Mutant lane shows a 121 nucleotide band lacking 67 nucleotides of exon 2. DNA marker lane shows 400, 300, 200 and 100 bp bands. (E) Western blot showing absence of Lef1 band in lef1^u767 mutant. (F-M) Expression of lef1 (F,G), tcf7 (H,I), tcf7l1a (J,K) and tcf7l2 (L,M) in wild-type (F,H,J,L) and lef1^u767 mutant (G,I,K,M) 33 hpf embryos. Primordia are outlined. (N-Q) GFP expression in control Tg(7Xtcf-siam:GFP)^ia4 Wnt reporter (N), lef1 morphant (O), BIO incubated (P) and BIO-incubated/lef1-morphant (Q) 36 hpf embryos. PLLP is outlined in each case. Brackets indicate the length of the primordium.