FIGURE

Fig. S7

ID

ZDB-FIG-110915-30

Publication

Totong et al., 2011 - The novel transmembrane protein Tmem2 is essential for coordination of myocardial and endocardial morphogenesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. S7

Myocardial expression of tmem2 does not rescue frv mutants. (A-F) Frontal views depicting expression of AVC markers at 48 hpf (as in Fig. 1). All embryos shown carry the Tg(myl7:tmem2-gfp) transgene, as confirmed by examination of fluorescence prior to fixation. (A-C) Expression of Tg(myl7:tmem2-gfp) does not alter the phenotype of wt embryos (compare with Fig. 1I-K). (D-F) Expression of Tg(myl7:tmem2-gfp) does not alter the phenotype of frv mutant embryos (compare with Fig. 1L-N). (G-I) Three-dimensional projections of selected confocal sections of an frv mutant heart expressing Tg(myl7:tmem2-gfp) (green) in the myocardium at 57 hpf. Immunofluorescence reveals no effect of the transgene on ectopic localization of Dm-grasp (red) (compare with Fig. 1R-T). Comparable results were obtained with two independent transgenic lines. Scale grids are 23 μm per segment. Scale bar: 50 μm.

Expression Data

Genes:	alcama bmp4 EGFP notch1b vcana
Antibody:	zn-5
Fish:	sd13Tg cemip2^sk38/sk38; sd13Tg
Anatomical Terms:	atrioventricular canal atrium cardiac ventricle ventricular endocardium ventricular myocardium
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cemip2^sk38/sk38; sd13Tg
Observed In:	atrioventricular canal atrioventricular valve formation atrium cardiac ventricle cardiocyte differentiation ventricular endocardium
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development