FIGURE

Fig. 4

ID

ZDB-FIG-110915-24

Publication

Totong et al., 2011 - The novel transmembrane protein Tmem2 is essential for coordination of myocardial and endocardial morphogenesis

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Fig. 4

Maternal-zygotic frv mutants exhibit myocardial and endocardial fusion defects that are rescued by myocardial tmem2 expression. (A,B) Lateral views of zebrafish maternal (Mfrv) and maternal-zygotic (MZfrv) frv mutant siblings reveal body length and yolk extension defects in MZfrv embryos at 24 hpf. (C,D,G-J) Dorsal views depict myl7 expression at 20 somites (som) and 26 hpf. (E,F) Dorsal views depict endocardial expression of Tg(kdrl:GRCFP) (arrowheads) at 24 hpf. (K-N) Dorsal views depict endocardial expression of cdh5 (arrows) at 28 hpf In Mfrv embryos, expression of Tg(myl7:tmem2-gfp) has no effect on myocardial or endocardial morphogenesis (G,H,K,L), whereas in MZfrv embryos expression of Tg(myl7:tmem2-gfp) rescues both myocardial and endocardial fusion (I,J,M,N). Non-transgenic (non-tg) and transgenic siblings were distinguished by examination of fluorescence before fixation. Comparable results were obtained with two independent transgenic founders. Scale bars: 50 μm.

Expression Data

Genes:	cdh5 grcfp myl7
Fish:	cemip2^sk38/sk38 cemip2^sk38/+ cemip2^sk38/+; zf528Tg cemip2^sk38/sk38; zf528Tg cemip2^sk38/+; sd13Tg cemip2^sk38/sk38; sd13Tg
Anatomical Terms:	endocardium heart primordium heart rudiment myocardium
Stage Range:	20-25 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cemip2^sk38/sk38 cemip2^sk38/+ cemip2^sk38/+; zf528Tg cemip2^sk38/sk38; zf528Tg cemip2^sk38/+; sd13Tg cemip2^sk38/sk38; sd13Tg
Observed In:	cardiac muscle progenitor cell migration to the midline involved in heart field formation endocardial progenitor cell migration to the midline involved in heart field formation endocardium extension myocardium trunk
Stage Range:	20-25 somites to Prim-5

Phenotype Detail

Acknowledgments

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