Fig. 7
- ID
- ZDB-FIG-110909-84
- Publication
- Chang et al., 2011 - liver-enriched gene 1a and 1b Encode Novel Secretory Proteins Essential for Normal Liver Development in Zebrafish
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Hypoplastic liver in leg1-MOATG morphants is caused by cell cycle arrest during the liver budding stage. (A, B) Immunostaining using an anti-PH3 antibody on st-MO (A) and ATG-MO (B) morphants at 38hpf. Liver primordium is circled with a white dotted line. (C, D) Immunostaining using antibodies respectively against PH3 (red) and RFP (green) on cross-sections from st-MO (C) and ATG-MO (D) morphants in the Tg(lfabp: DsRed; elaA: EGFP) background at 3dpf. (E) Statistical analysis revealed that the ratio of PH3-positive cells was significantly reduced in the liver primordium of ATG-MO morphants at 38 hpf, but no significant difference in the ratio of PH3-positive cells was observed in the liver of ATG-MO morphants at 3dpf. Data was obtained by counting PH3-positive cells versus total cells in a specific organ (e.g. liver) in sections from at least 3 st-MO embryos and 3 ATG-MO morphants at each stage, respectively (Table S1). |
| Genes: | |
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| Fish: | |
| Knockdown Reagent: | |
| Anatomical Terms: | |
| Stage Range: | Prim-15 to Long-pec |
| Fish: | |
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| Knockdown Reagent: | |
| Observed In: | |
| Stage: | Prim-25 |