FIGURE

Fig. 3

ID

ZDB-FIG-110920-102

Publication

Chang et al., 2011 - liver-enriched gene 1a and 1b Encode Novel Secretory Proteins Essential for Normal Liver Development in Zebrafish

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Fig. 3

leg1a and leg1b are differentially expressed.

(A) Alignment of 5′- and 3′-UTRs of leg1a and leg1b transcripts, respectively. Asterisk highlights identical bases. Translation start codon ATG and stop codon TGA are lettered in red. (B) Comparison of leg1a and leg1b expression at different developmental stages as indicated. Data are presented in percentage (blue bar: leg1a; red bar: leg1b). (C) Anti-Leg1 monoclonal antibody recognizes both Leg1a and Leg1b which were induced to express in E. coli by isopropyl β-D-1-thiogalactopyranoside (IPTG). (D) Western blotting analysis of total Leg1 (Leg1a+Leg1b) expression in embryos at stages as indicated.

Expression Data

Genes:	leg1.1 leg1.2
Fish:	AB
Anatomical Terms:	liver whole organism
Stage Range:	Prim-5 to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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