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ZIRC
ZFIN ID: ZDB-FIG-110907-27
Lou et al., 2011 - Smarcd3b and Gata5 promote a cardiac progenitor fate in the zebrafish embryo. Development (Cambridge, England)   138(15):3113-23 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 5-9 somites to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 5-9 somites to Long-pec

Fig. 1

Simultaneous inhibition of cBAF complex components leads to defects in myocardial specification. Suboptimal (1 ng of each MO in all cases) levels of morpholinos targeting smarcd3b, gata5 and tbx5 were injected as indicated. (A-H) RNA in situ hybridization for nkx2.5 expression at the eight-somite stage (13 hpf). Dorsal views with anterior towards the top of images. (I-P) RNA in situ hybridization for myl7 expression at 24 hpf. Dorsal views with anterior towards the bottom of images. (Q-X) Fluorescent images of 48 hpf hearts using the myocardial-specific myl7:EGFP transgene. Ventral views are shown with anterior towards the top of images.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP twu34Tg control Long-pec atrial myocardium IFL
Long-pec ventricular myocardium IFL
twu34Tg + MO1-gata5 standard conditions Long-pec atrial myocardium IFL
Long-pec ventricular myocardium IFL
twu34Tg + MO1-gata5 + MO1-smarcd3b standard conditions Long-pec cardiac muscle cell IFL
twu34Tg + MO1-gata5 + MO1-smarcd3b + MO1-tbx5a standard conditions Long-pec cardiac muscle cell IFL
twu34Tg + MO1-gata5 + MO1-tbx5a standard conditions Long-pec cardiac muscle cell IFL
twu34Tg + MO1-smarcd3b standard conditions Long-pec atrial myocardium IFL
Long-pec ventricular myocardium IFL
twu34Tg + MO1-smarcd3b + MO1-tbx5a standard conditions Long-pec atrial myocardium IFL
Long-pec ventricular myocardium IFL
twu34Tg + MO1-tbx5a standard conditions Long-pec atrial myocardium IFL
Long-pec ventricular myocardium IFL
myl7 WT control Prim-5 heart tube ISH
WT + MO1-gata5 standard conditions Prim-5 heart tube ISH
WT + MO1-gata5 + MO1-smarcd3b standard conditions Prim-5 cardiac muscle cell ISH
WT + MO1-gata5 + MO1-smarcd3b + MO1-tbx5a standard conditions Prim-5 cardiac muscle cell ISH
WT + MO1-gata5 + MO1-tbx5a standard conditions Prim-5 cardiac muscle cell ISH
WT + MO1-smarcd3b standard conditions Prim-5 heart tube ISH
WT + MO1-smarcd3b + MO1-tbx5a standard conditions Prim-5 heart tube ISH
WT + MO1-tbx5a standard conditions Prim-5 heart tube ISH
nkx2.5 WT control 5-9 somites heart primordium ISH
WT + MO1-gata5 standard conditions 5-9 somites heart primordium ISH
WT + MO1-gata5 + MO1-smarcd3b standard conditions 5-9 somites heart primordium ISH
WT + MO1-gata5 + MO1-smarcd3b + MO1-tbx5a standard conditions 5-9 somites Not Detected heart primordium ISH
WT + MO1-gata5 + MO1-tbx5a standard conditions 5-9 somites heart primordium ISH
WT + MO1-smarcd3b standard conditions 5-9 somites heart primordium ISH
WT + MO1-smarcd3b + MO1-tbx5a standard conditions 5-9 somites heart primordium ISH
WT + MO1-tbx5a standard conditions 5-9 somites heart primordium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-gata5 standard conditions 5-9 somites heart morphogenesis process quality, normal
WT + MO1-gata5 standard conditions Prim-5 cardiac muscle cell normal amount, normal
WT + MO1-gata5 standard conditions Prim-5 heart morphology, normal
WT + MO1-gata5 + MO1-smarcd3b standard conditions 5-9 somites heart morphogenesis disrupted, abnormal
Prim-5 cardiac muscle cell decreased amount, abnormal
Prim-5 cardiac muscle cell differentiation disrupted, abnormal
Prim-5 heart tube bifurcated, abnormal
WT + MO1-gata5 + MO1-smarcd3b + MO1-tbx5a standard conditions 5-9 somites heart morphogenesis arrested, abnormal
Prim-5 cardiac muscle cell decreased amount, abnormal
Prim-5 cardiac muscle cell differentiation disrupted, abnormal
Prim-5 heart tube bifurcated, abnormal
WT + MO1-gata5 + MO1-tbx5a standard conditions 5-9 somites heart morphogenesis disrupted, abnormal
Prim-5 cardiac muscle cell decreased amount, abnormal
Prim-5 cardiac muscle cell differentiation disrupted, abnormal
Prim-5 heart tube bifurcated, abnormal
WT + MO1-smarcd3b standard conditions 5-9 somites heart morphogenesis process quality, normal
Prim-5 cardiac muscle cell normal amount, normal
Prim-5 heart morphology, abnormal
WT + MO1-smarcd3b + MO1-tbx5a standard conditions 5-9 somites heart morphogenesis disrupted, abnormal
Prim-5 cardiac muscle cell normal amount, normal
Prim-5 heart morphology, abnormal
WT + MO1-tbx5a standard conditions 5-9 somites heart morphogenesis process quality, normal
Prim-5 cardiac muscle cell normal amount, normal
Prim-5 heart morphology, normal
twu34Tg + MO1-gata5 standard conditions Long-pec cardiac muscle cell differentiation process quality, normal
Long-pec heart looping process quality, normal
twu34Tg + MO1-gata5 + MO1-smarcd3b standard conditions Long-pec cardiac muscle cell differentiation disrupted, abnormal
Long-pec heart bifurcated, abnormal
Long-pec heart morphogenesis disrupted, abnormal
twu34Tg + MO1-gata5 + MO1-smarcd3b + MO1-tbx5a standard conditions Long-pec cardiac muscle cell differentiation disrupted, abnormal
Long-pec heart bifurcated, abnormal
Long-pec heart morphogenesis disrupted, abnormal
twu34Tg + MO1-gata5 + MO1-tbx5a standard conditions Long-pec cardiac muscle cell differentiation disrupted, abnormal
Long-pec heart bifurcated, abnormal
Long-pec heart morphogenesis disrupted, abnormal
twu34Tg + MO1-smarcd3b standard conditions Long-pec cardiac muscle cell differentiation process quality, normal
Long-pec heart looping disrupted, abnormal
twu34Tg + MO1-smarcd3b + MO1-tbx5a standard conditions Long-pec cardiac muscle cell differentiation process quality, normal
Long-pec heart looping disrupted, abnormal
twu34Tg + MO1-tbx5a standard conditions Long-pec cardiac muscle cell differentiation process quality, normal
Long-pec heart looping process quality, normal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development