FIGURE

Fig. 2

ID

ZDB-FIG-110811-46

Publication

Webb et al., 2011 - The Enhancer of split transcription factor Her8a is a novel dimerisation partner for Her3 that controls anterior hindbrain neurogenesis in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Embryonic expression of her8a. Expression is revealed by whole-mount in situ hybridization at the stages indicated (som: somites, hpf: hours post-fertilization) together with other positional marker genes (B,C,E, color-coded) or proteins (G, immunocytochemistry for the neuronal marker HuC/D in green). A-E and H are dorsal views of flat-mounted embryos, F,I are lateral views, all embryos are viewed anterior left. G is a cross section of a 24hpf embryo at midbrain levels (as indicated in F). At early neural plate stages, the domain of strongest her8a expression covers the mid- and anterior hindbrain (bracket in A-C). It overlaps the presumptive midbrain-hindbrain boundary (her5-positive, purple arrow in C) and the progenitor pools separating medial and lateral hindbrain neurons (her3-positive, purple arrow in B). It extends into rhombomere 2 (white arrowhead in C), more posterior rhombomeres being more weakly labeled. At 10 somites, expression in the midbrain is maintained. It resolves in stripes in the rhombencephalon (D,E). It avoids the midbrain-hindbrain boundary and zona limitans intrathalamica (asterisk). From 24 hpf onwards, her8a characterizes the ventricular zone and progenitor domains of the neural tube (G-I, arrows in I point to the ventricular zone and the arrowhead points to the progenitor domain of the optic tectum). Abbreviations: e: eye field, mid: presumptive midbrain, mhb: midbrain-hindbrain boundary, r: rhombomere. Scale bars: 100 μm.

Expression Data

Genes:	her3 her5 her8a
Fish:	WT
Anatomical Terms:	brain ventricular zone hindbrain hindbrain neural keel hindbrain neural keel anterior region midbrain midbrain hindbrain boundary neural plate midbrain neural keel neural tube ventricular zone optic tectum neuronal stem cell presumptive rhombomere 1 presumptive rhombomere 2 rhombomere 1 rhombomere 3 rhombomere 5
Stage Range:	Bud to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.