ZFIN ID: ZDB-FIG-110706-34
Trikić et al., 2011 - Regulation of Zebrafish Hatching by Tetraspanin cd63. PLoS One   6(5):e19683 Full text @ PLoS One
Knockdown Reagent:
Anatomical Terms:
Stage Range: 75%-epiboly to Prim-5

Fig. 6

Determination of mesodermal markers by ISH.

Morphant embryos were injected with 10 pg MO4. A. Location of pre-chordal plate marker gsc in LWT embryos at 80% epiboly. I and II give lateral view, III and IV looking down on gsc positive staining. II and IV were injected with MO4, I and III are mis-match injected siblings. Arrows indicate gsc specific staining. B. Hatching gland marker cat L in 24 hour post fertilisation LWT embryos. I and II are morphant embryos, ventral and lateral views respectively. III and IV are mismatch injected LWT embryos, ventral and lateral views respectively. Arrows indicate hatching gland specific cat L staining.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ctslb WT control Prim-5 hatching gland ISH
WT + MO3-cd63 standard conditions Prim-5 hatching gland ISH
gsc WT control 75%-epiboly prechordal plate ISH
WT + MO3-cd63 standard conditions 75%-epiboly prechordal plate ISH
Antibody Labeling Details No data available
Phenotype Details No data available
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