|ZFIN ID: ZDB-FIG-110706-34|
|Stage Range:||75%-epiboly to Prim-5|
Determination of mesodermal markers by ISH.
Morphant embryos were injected with 10 pg MO4. A. Location of pre-chordal plate marker gsc in LWT embryos at 80% epiboly. I and II give lateral view, III and IV looking down on gsc positive staining. II and IV were injected with MO4, I and III are mis-match injected siblings. Arrows indicate gsc specific staining. B. Hatching gland marker cat L in 24 hour post fertilisation LWT embryos. I and II are morphant embryos, ventral and lateral views respectively. III and IV are mismatch injected LWT embryos, ventral and lateral views respectively. Arrows indicate hatching gland specific cat L staining.
|WT + MO3-cd63||standard conditions||Prim-5||hatching gland||ISH|
|WT + MO3-cd63||standard conditions||75%-epiboly||prechordal plate||ISH|
|Acknowledgments:||ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One|