FIGURE

Fig. 4

ID

ZDB-FIG-110913-2

Publication

Trikić et al., 2011 - Regulation of Zebrafish Hatching by Tetraspanin cd63

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Fig. 4

Effect of knockdown.

A. Effect of morpholino-induced knockdown of cd63 on hatching. Buff = buffer/mismatch injected control. Units of time are hours. Data from two experiments each using MO2 and 4 and a combined total of 671 embryos. B. MO4 mediated band shift. 1% Agarose gel of RT-PCR products from 48 hr LWT embryos using primers corresponding to full length cd63 transcript or ef1, a ubiquitous transcription factor used as a control. The 3 lanes next to the ladder are from embryos injected with 10 pg MO4, remaining lanes are from mismatch MO3 injected embryos. Similar results were seen in the 30 MO4 injected embryos tested.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-cd63 WT + MO3-cd63
Knockdown Reagents:	MO2-cd63 MO3-cd63
Observed In:	hatching
Stage Range:	Prim-15 to Day 4

Phenotype Detail

Acknowledgments

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