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ZFIN ID: ZDB-PUB-110609-30
Regulation of Zebrafish Hatching by Tetraspanin cd63
Trikić, M.Z., Monk, P., Roehl, H., and Partridge, L.J.
Date: 2011
Source: PLoS One   6(5): e19683 (Journal)
Registered Authors: Roehl, Henry
Keywords: Embryos, Zebrafish, Lysosomes, Morpholino, Cats, Fertilization, Polymerase chain reaction, Reverse transcriptase-polymerase chain reaction
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Embryo, Nonmammalian/cytology*
  • Embryo, Nonmammalian/physiology*
  • Models, Animal
  • Molecular Sequence Data
  • Tetraspanin 30/metabolism*
  • Zebrafish/physiology*
PubMed: 21625559 Full text @ PLoS One
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ABSTRACT
Tetraspanins cause the clustering of membrane proteins into a level of organisation essential for cellular function. Given the importance and complicated nature of this mechanism, we attempted a novel approach to identify the function of a single component in a biologically relevant context. A morpholino knockdown strategy was used to investigate the role of cd63, a membrane protein associated with intracellular transport and a melanoma marker, in embryonic zebrafish. By using three separate morpholinos targeting cd63, we were able to identify a specific phenotype. Strikingly, morphant fish failed to hatch due to the lack of secreted proteolytic enzymes required for chorion-softening. The morphology of the hatching gland at both the cellular and intracellular levels was disorganised, suggesting a role for cd63 in the functioning of this organ. This work identifies a specific role for cd63 in the zebrafish embryo and provides evidence for the suitability of zebrafish as a model system for the investigation of tetraspanin enriched microdomains.
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