FIGURE

Fig. s3

ID

ZDB-FIG-110621-17

Publication

Langenbacher et al., 2011 - The PAF1 complex differentially regulates cardiomyocyte specification

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Fig. s3

Morpholinos targeting PAF1C component genes reduce target protein levels. (A–D) Western blots detecting PAF1C component proteins and β-actin protein levels in uninjected control and morpholino injected embryo lysates. (A) Ctr9 protein is reduced to 38% of control levels in ctr9MO injected embryos. (B) Paf1 protein is reduced to 43% of control levels in paf1MO injected embryos. (C) Cdc73 protein is reduced to 39% of control levels in cdc73MO injected embryos. (D) Rtf1 protein is reduced to 7% of control levels in rtf1MO injected embryos.

Expression Data

Genes:	cdc73 ctr9 paf1 rtf1
Antibodies:	Ab1-cdc73 Ab1-ctr9 Ab1-paf1 Ab1-rtf1
Fish:	WT WT + MO1-ctr9 WT + MO1-rtf1 WT + MO2-cdc73 WT + MO2-paf1
Knockdown Reagents:	MO1-ctr9 MO1-rtf1 MO2-cdc73 MO2-paf1
Anatomical Term:	whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 353(1), Langenbacher, A.D., Nguyen, C.T., Cavanaugh, A.M., Huang, J., Lu, F., and Chen, J.N., The PAF1 complex differentially regulates cardiomyocyte specification, 19-28, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.