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ZFIN ID: ZDB-FIG-110621-9
Langenbacher et al., 2011 - The PAF1 complex differentially regulates cardiomyocyte specification. Developmental Biology   353(1):19-28 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Prim-15

Fig. 1

Fig. 1. Mutation in zebrafish ctr9 perturbs primitive heart tube elongation and causes a reduction in cardiomyocyte cell number. (A and B) Lateral views of 24 hpf wild type (A) and LA961 mutant (B) embryos. LA961 mutants can be distinguished from wild type embryos based on missing otoliths (arrowhead) and pericardial edema (arrow). (C and D) Wild type and LA961 mutant embryos analyzed for cmlc2 expression at 24 hpf. Wild type embryos have an elongated primitive heart tube (C), whereas LA961 mutants have a clump of cardiomyocytes at the midline (D). (E) Graph of number of GFP-positive cardiomyocytes in wild type (WT) and LA961 mutant embryos. Error bars indicate standard deviations. (F) Lateral view of wild type (top) and LA961 mutant (bottom) embryos at 35 hpf. (G) Diagram of LA961 mapping. Estimated genetic distance is in centimorgans (cM). (H) Diagram of Ctr9WT and Ctr9LA961 proteins. Grey regions are predicted tetratricopeptide repeat domains and black regions are predicted nuclear localization domains.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ctr9la961/la961; twu34Tg standard conditions Prim-5 cardiac muscle cell IFL
twu34Tg standard conditions Prim-5 cardiac muscle cell IFL
myl7 WT control Prim-5 heart tube ISH
ctr9la961/la961(AB) control Prim-5 cardiac muscle cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ctr9la961/la961(AB) standard conditions Prim-5 embryonic heart tube morphogenesis disrupted, abnormal
Prim-5 heart tube aplastic, abnormal
Prim-5 inner ear lacks all parts of type otolith, abnormal
Prim-5 pericardium edematous, abnormal
Prim-15 embryonic heart tube morphogenesis disrupted, abnormal
Prim-15 heart tube hypoplastic, abnormal
Prim-15 heart tube malformed, abnormal
Prim-15 pigment cell absent, abnormal
Prim-15 whole organism curved ventral, abnormal
ctr9la961/la961; twu34Tg standard conditions Prim-5 cardiac muscle cell decreased amount, abnormal
Prim-5 cardiac muscle cell differentiation decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 353(1), Langenbacher, A.D., Nguyen, C.T., Cavanaugh, A.M., Huang, J., Lu, F., and Chen, J.N., The PAF1 complex differentially regulates cardiomyocyte specification, 19-28, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.