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ZFIN ID: ZDB-FIG-110531-11
Liu et al., 2011 - Tumor suppressor Lzap regulates cell cycle progression, doming, and zebrafish epiboly. Developmental dynamics : an official publication of the American Association of Anatomists   240(6):1613-1625 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Shield to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Sphere to 90%-epiboly

Fig. 5 Depletion of Lzap causes delay in cell cycle progression but does not disrupt zygotic gene expression after midblastula transition (MBT). A: The expression of bmp2b, eve1, ntl, and hgg1 in control and both age-matched and stage-matched lzap morphant embryos were detected by whole-mount in situ hybridization. bmp2b expression, lateral view, 6 hours postfertilization (hpf) in control. eve1 and ntl expression, top view, 6 hpf in control. hgg1 expression, lateral view, 12 hpf in control. B: Cell cycle analyses of dissociated zebrafish embryonic cells. DNA content was determine on untreated embryonic cells (a), on cells treated with 10 μg/ml nocodazole for 30 min (b), or on cells treated with nocodazole followed by release for 15 (c) or 30 min (d). Cells were assigned to G1, S, or G2/M based on fluorescent signal and manual gating using CellQuest Pro software. Results in the table represent the mean of at least three independent experiments. C: Embryos with and without injection of 4 ng of lzap morpholino (MO) were co-immunostained with anti–α-tubulin (red) and p-Histone H3 (green) antibodies at 4, 6, and 9 hpf in mitotic cells. Results are representative of observations from more than 100 nuclei for each panel.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
bmp2b AB control Shield blastoderm ISH
AB + MO1-cdk5rap3 standard conditions Shield blastoderm ISH
90%-epiboly blastoderm ISH
ctslb AB control 5-9 somites polster ISH
AB + MO1-cdk5rap3 standard conditions Prim-5 polster ISH
eve1 AB control Shield margin ISH
AB + MO1-cdk5rap3 standard conditions Shield blastoderm ISH
90%-epiboly margin ISH
tbxta AB control Shield margin ISH
AB + MO1-cdk5rap3 standard conditions Shield blastoderm ISH
90%-epiboly margin ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-cdk5rap3 standard conditions Sphere G2/M transition of mitotic cell cycle delayed, abnormal
Sphere mitotic cell cycle delayed, abnormal
Sphere - 90%-epiboly blastoderm cell chromatin morphology, normal
Sphere - 90%-epiboly blastoderm cell chromosome orientation blastoderm cell chromosome, normal
Sphere - 90%-epiboly mitotic chromosome condensation process quality, normal
Sphere - 90%-epiboly spindle assembly process quality, normal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.