ZFIN ID: ZDB-FIG-110527-5
Krueger et al., 2011 - Flt1 acts as a negative regulator of tip cell formation and branching morphogenesis in the zebrafish embryo. Development (Cambridge, England)   138(10):2111-2120 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-15 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 5 Distribution of Flt1 in vessels and nerves. (A) Comparison of flt1BAC:yfp and kdrl:cherry expression in segmental artery sprouts of 30 hpf Tg(kdrl:ras-cherry)s916 × Tg(flt1BAC:yfp) double-transgenic zebrafish embryos, in controls (top row) and flt1 morphants (bottom row). In controls, note the overlap of flt1BAC:yfp and kdrl:cherry expression in tip and stalk cells. At 30 hpf, expression of flt1BAC:yfp is restricted to the vascular compartment. After injection of flt1 ATG-blocking MO, expression of flt1BAC:yfp in sprouts appears less intense, but is not completely lost. (B) Injection of flt1 ATG-blocking MO in Tg(kdrl:ras-cherry)s916 × Tg(flt1BAC:yfp) double-transgenic embryos causes hyperbranching of segmental vessels and affects neurons. In controls at 48 hpf, flt1BAC:yfp marks segmental vessels and a subset of spinal cord neurons (arrowhead). Injection of the flt1 ATG-blocking MO induces aberrant vessel patterning and the neuronal flt1BAC:yfp expression domain is reduced. Injection of the flt1 ATG-blocking MO in Tg(kdrl:hras-mcherry)s896 × Tg(huC:egfp) double-transgenic embryos affects neurons. (C-E3) Flt1 immunostaining in Tg(huC:egfp) neuronal reporter embryos shows Flt1 throughout the neural tube. Overview of neural tube (C-C3) and different focal planes from dorsal to ventral (D-E3) are shown. (F) Flt1 immunostaining in Tg(flt1BAC:yfp) embryos shows co-localization of flt1BAC:yfp with Flt1 antibody staining in spinal cord neurons (boxed). (G) Immunostaining for Flt1 and zn-12 shows Flt1 staining of Rohon-Beard sensory neurons. (H) Overexpression of fli1ep:cherry-sflt1 in Tg(huC:egfp) embryos results in neuronal sFlt1-cherry expression throughout the neural tube. Arrowheads indicate co-localization of huC:egfp with sFlt1-cherry on neuronal cell bodies. (I) Overexpression of kdrl:egfp-sflt1 in Tg(kdrl:hras-mcherry)s896 embryos shows sFlt1-egfp expression in vessels and the neural tube (arrowheads). Nt, neural tube; Se, segmental artery.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP knu3Tg standard conditions Prim-15 spinal cord motor neuron IFL
Prim-15 spinal cord sensory neuron IFL
Prim-15 spinal cord interneuron IFL
knu3Tg; s896Tg standard conditions Long-pec spinal cord neuron IFL
knu3Tg; s896Tg + MO3-flt1 standard conditions Long-pec spinal cord neuron IFL
y1Tg standard conditions Prim-15 dorsal aorta IFL
Prim-15 intersegmental vessel IFL
Prim-15 Rohon-Beard neuron IFL
flt1 hu4624Tg standard conditions Prim-25 spinal cord neuron IHC
knu3Tg standard conditions Prim-15 spinal cord motor neuron IHC
Prim-15 spinal cord sensory neuron IHC
Prim-15 spinal cord interneuron IHC
mCherry hu4624Tg; s916Tg standard conditions Prim-15 dorsal aorta IFL
Prim-15 intersegmental vessel IFL
Prim-15 intersegmental vessel endothelial tip cell IFL
Long-pec dorsal aorta IFL
hu4624Tg; s916Tg + MO3-flt1 standard conditions Prim-15 dorsal aorta IFL
Prim-15 intersegmental vessel IFL
Prim-15 intersegmental vessel endothelial tip cell IFL
Long-pec dorsal aorta IFL
knu3Tg; s896Tg standard conditions Long-pec dorsal aorta IFL
Long-pec dorsal longitudinal anastomotic vessel IFL
Long-pec intersegmental vessel IFL
knu3Tg; s896Tg + MO3-flt1 standard conditions Long-pec dorsal aorta IFL
Long-pec dorsal longitudinal anastomotic vessel IFL
Long-pec intersegmental vessel IFL
YFP hu4624Tg standard conditions Prim-25 dorsal longitudinal anastomotic vessel IFL
Prim-25 intersegmental vessel IFL
Prim-25 spinal cord neuron IFL
hu4624Tg; s916Tg standard conditions Prim-15 dorsal aorta IFL
Prim-15 intersegmental vessel IFL
Prim-15 intersegmental vessel endothelial tip cell IFL
Long-pec intersegmental vessel IFL
Long-pec spinal cord neuron IFL
hu4624Tg; s916Tg + MO3-flt1 standard conditions Prim-15 dorsal aorta IFL
Prim-15 intersegmental vessel IFL
Prim-15 intersegmental vessel endothelial tip cell IFL
Long-pec intersegmental vessel IFL
Long-pec spinal cord neuron IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zn-12 IHC y1Tg standard conditions Prim-15 Rohon-Beard neuron
Phenotype Details
Fish Conditions Stage Phenotype
hu4624Tg; s916Tg + MO3-flt1 standard conditions Long-pec intersegmental artery branched, abnormal
knu3Tg; s896Tg + MO3-flt1 standard conditions Long-pec spinal cord has fewer parts of type CNS neuron (sensu Vertebrata), abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development